嗜酸乳杆菌NCFM粘附宿主后对其粘附相关基因表达的影响

摘要：【目的】益生菌粘附于肠道上皮细胞上是它的一种益生作用。本研究通过体内外实验，分析嗜酸乳杆菌NCFM对粘附相关基因的影响。【方法】利用GO (Gene Ontolog) 分类筛选Human Genome U133 Plus 2.0 Array基因表达谱芯片中的粘附相关基因，通过体外Caco-2细胞培养模型和体内小鼠粘附模型，采用Real-time PCR方法对粘附相关基因进行验证分析。【结果】经NCFM作用后，12个粘附相关基因呈上调表达。利用Real-time PCR验证，12个基因在体内和体外经嗜酸乳杆菌NCFM作用后亦均同样为上调表达，其中CCL2基因上调表达最为明显。【结论】经体内外研究表明，嗜酸乳杆菌NCFM粘附肠上皮细胞后能够引起宿主粘附相关基因出现特定表达变化，为今后深入揭示其粘附作用提供必要基础。

Lactobacillus acidophilus NCFM affects host adhesion-related gene expression after adhering to host

Abstract: [Objective] Adhering to the intestinal epithelial cells is one of the beneficial functions exerting by probiotics. We tested and verified the expression of adhesion-related genes in vivo and in vitro to understand the influence from Lactobacillus acidophilus NCFM on host cells. [Method] We selected the adhesion-related genes through GO (gene ontology) category from the Human Genome U133 Plus 2.0 Array analysis. These genes were verified by in vitro Caco-2 cells culture model and in vivo mouse model using Real-time PCR method. [Results] After L. acidophilus NCFM adhering to Caco-2, we found that 12 adhesion-related genes were up-regulated. The up-regulation was confirmed in vivo and in vitro by Real-time PCR assay. Among them, the up-regulated expression of chemokine (C-C motif) ligand 2 (CCL2) gene was the most distinguished one. [Conclusion] Our research showed that L. acidophilus NCFM, adhering to Caco-2 cells, could cause the differential expression of the host adhesion-related genes. Results of this study seemed to provide some useful data for further revealing its effect on intestinal epithelial cells.