脂联素基因多态性与2型糖尿病肾病的关系

目的研究脂联素(APN)基因+45位点与+276位点单核苷酸多态性(SNP)与中国浙江地区汉族人群2型糖尿病肾病(DN)的关系。方法根据尿白蛋白排泄率(UAER)将82例2型糖尿病患者分成糖尿病正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组;用直接测序法分析SNP+45和+276两位点基因型和等位基因频率,并分析与其他指标的关系。结果脂联素基因SNP+45和+276两多态性位点的基因型和等位基因频率分别在正常白蛋白尿组、微量白蛋白尿组和大量白蛋白尿组3组间无统计学差异(P>0.05)。结论脂联素基因SNP+45T/G和SNP+276G/T与浙江地区汉族人群中2型糖尿病肾病无相关性。这两位点的基因多态性不能预测2型糖尿病肾病的易感性。

Association of Adiponectin Gene Polymorphism in Type 2 Diabetes with Nephropathy

Objective To investigate the association of single nucleotide polymorphism(SNP) of +45 and +276 loci of adiponectin(APN)gene with type 2 diabetic nephropathy(DN) in Han ethnicity population,Zhejiang province in China.Methods Eighty-two type 2 diabetic patients were assigned to normal albuminuria group,micro-albuminuria group,and macro-albuminuria group according to the urine albumin excretion rate(UAER).Direct sequencing was used to analyze allele and genotype frequencies of SNP+45 and +276 loci,and analying the relationship with other observed indicators.Results The distributions of genotype and allele frequencies of SNP+45 and +276 had no difference among three subgroups(P>0.05).Conclusion The T→G polymorphism of SNP+45 and G→T polymorphism of SNP+276 in adiponectin gene are not associated with type 2 diabetic nephropathy in Han ethnicity population,Zhejiang province in China.The gene polymorphisms of these two loci can not predict the susceptibility to type 2 diabetic nephropathy.