百令胶囊对糖尿病肾病大鼠生化指标及肾组织病理学改变的影响

目的:观察百令胶囊对糖尿病肾病大鼠血压、血糖、24 h尿蛋白定量、肾功能及大鼠肾组织病理变化的作用.方法:24只大鼠随机分为对照组、模型组、治疗组,每组各8只.复制糖尿病肾病大鼠模型成功2 d后,治疗组每日灌胃百令胶囊(2.5 g/kg),模型组及对照组每日给予等量的饮用水,测定血糖及24 h尿蛋白定量;通过全自动生化分析仪检测血肌酐(Scr)、尿素氮(BUN),并计算内生肌酐清除率(Ccr);通过光镜观察肾脏病理变化.结果:给药12周后,模型组大鼠血糖、平均动脉压、24 h尿蛋白定量、Scr、BUN及左肾/体重均明显高于对照组大鼠,而Ccr低于对照组大鼠,均P＜0.05,且肾组织出现了较明显的病理损伤;经百令胶囊治疗后,大鼠平均动脉压、24 h尿蛋白定量、Scr、BUN及左肾/体重均明显减低,均P＜0.05,血糖减低,Ccr显著增高,P＜0.05,肾组织病理损伤明显减轻.结论:百令胶囊对糖尿病肾病大鼠具有肾保护作用.

Effect of Bailin Capsule on Biochemical Indexs and Renal Histopathological Changes in Rats with Diabetic Nephropathy

Objective:To observe the effect of Bailin capsule on blood pressure,blood sugar,urinary protein/24 h,renal function,pathological changes of renal tissue in rats with diabetic nephropathy. Methods:24 rats were randomly grouped into control,model and treatment,8 rats for each group. Two days after models' establishment,Bailin capsule was administrated (2.5 g/kg) by a stomach tube in treatment group,and same amount of water was fed in model and control group. Then we detected the blood sugar,urinary protein/24 h,serum creatinine (Scr),blood urea nitrogen (BUN) .calculated endogenous creatinine clearance rate (Ccr), and used optics microscope and electronic microscope to observe the the renal pathological changes. Results: After 12 w,the blood sugar, average arterial pressure, urinary protein/24 h.Scr and BUN in model group were significantly higher compared with control group(respectively,P<0.05). However,Ccr in model group was lower than that of control group, and there was obvious renal tissue damage( respectively, P<0.05). After treatment of Bailin capsule, the average arterial pressure, urinary protein/24 h,Scr,BUN were reduced,Ccr increased and renal damage eased (respectively, P<0.05).Conclusion:Bailin treatment can protect diabetic nephropathy rat models from kidney damage.