玉米皮多糖的组成及结构

采用紫外光谱、纸层析及比色等分析方法对分离纯化制得的玉米皮多糖A-1(CSPA-1)、A-2(CSPA-2)、B(CSPB)的总糖含量、单糖组成及基本结构进行初步研究。结果表明：CSPA-1、CSPA-2 和CSPB 皆为白色粉末，其中CSPB 微溶于水，其他易溶于水，不溶于高浓度的有机溶剂，不含蛋白质和淀粉，均含有糖醛酸。CSPA-1总糖含量为99.3%，糖醛酸含量为16.6%，糖基组成为葡萄糖、木糖及阿拉伯糖，可能还含有微量鼠李糖；CSPA-2 总糖含量为93.4%，糖醛酸含量为21.7%，糖基组成为葡萄糖、木糖、阿拉伯糖及鼠李糖；CSPB 总糖含量为83.3%，糖醛酸含量为6.82%，糖基组成为葡萄糖、木糖及阿拉伯糖。高碘酸氧化分析表明：CSPA-1 中1 → 2 糖苷键或1 → 4 糖苷键残基比例为25%，1 → 6 糖苷键残基比例为3%；CSPA-2 中1 → 2 糖苷键或1 → 4 糖苷键残基比例为25.7%，1 → 6 糖苷键残基比例为3%；CSPB 中1 → 2 糖苷键或1 → 4 糖苷键残基比例为34.1%，1 → 6 糖苷键残基比例为1%。

Monosaccharide Component and Structure of Polysaccharides from Corn Spermoderm

The total polysaccharide content, monosaccharide component and structure of three factions of polysaccharides from corn spermoderm, CSPA-1, CSPA-2 and CSPB, were measured through the ultraviolet-visible spectrogram, paper chromatography and polarimetric analysis testing in this preliminary study. CSPA-1, CSPA-2 and CSPB all were white powder, and CSPB was only slightly soluble in water, while CSPA-1and CSPA-2 completely soluble. They all were insoluble in high concentrations of organic solvent and did not contain any protein or starch but glucuronic acid. Total polysaccharide content of CSPA-1 was 99.3%, and glucuronic acid content 16.6%. The monosaccharide unit of CSPA-1 was identified as glucose, xylose and arabinose, and possibly rhamnos. Total polysaccharide content of CSPA-2 was 93.4%, and glucuronic acid was 21.7%. The monosaccharide unit of CSPA-2 was identified as glucose, xylose, arabinose and rhamnose. Total polysaccharide content of CSPB was 83.3%, with glucuronic acid being 6.82%. The monosaccharide unit of CSPB was identified as glucose, xylose, and arabinose. Periodate oxidation analysis showed that CSPA-1 had 1 → 2 linked or 1 → 4 linked (25%) and 1 → 6 linked (3%); CSPA-2 was 1 → 2 linked or 1→4 linked (25.7%) and 1→6 linked (3%); CSPB was 1→2 linked or 1→4 linked (34.1%) and 1→6 linked (1%).