不同产地鲜姜乙醇浸提物化学成分分析及6-姜酚含量的测定

目的]分析比较不同产地鲜姜乙醇浸提物的化学成分并定量测定鲜姜中6-姜酚的含量。方法]采用GC-MS分析不同产地鲜姜乙醇浸提物中的化学成分,采用HPLC法测定鲜姜中6-姜酚的含量。结果]5个不同产地的鲜姜乙醇浸提物中分别鉴定出39、44、45、49、44种化合物,其主要成分为姜酚、姜烯酚类、姜酮酚类及姜油酮类等;由于产地不同,各产地鲜姜的乙醇浸提物组成成分及相对含量存在较大差异。姜酚类化合物在鲜姜中含量相对较高,约占总挥发油的30%左右。采用HPLC法测定鲜姜中6-姜酚的含量,结果显示,6-姜酚在0.024 8～0.124 mg/ml浓度范围内与峰面积呈良好线性关系（r=0.996 6,n=5）,平均加样回收率为99.20%,RSD为1.26%。结论]GC-MS法能快速、简便、高效的检测鲜姜乙醇浸提物的化学成分,HPLC测定法简便可靠、重复性好,可用于鲜姜中6-姜酚的含量测定。

Component Analysis on Ethanol Extract of RHIZOMA ZINGIBERIS RECENS from Different Producing Areas and Assay of 6-Gingerol

Objective] To analyze the chemical components in ethanol extracts of RHIZOMA ZINGIBERIS RECENS from different producing areas and to assay the content of 6-gingerol.Method] GC-MS analysis and HPLC method were respectively adopted to analyze the chemical components in ethanol extracts and assay the content of 6-gingerol.Result] 39,44,45,49 and 44 compounds were respectively identified from RHIZOMA ZINGIBERIS RECENS that was collected from 5 producing areas,mainly including gingerol(accounted for 30% of total essential oils),shogaols,paradols and zingerone.Because of different producing areas,there were significant differences regarding chemical components and their relative contents in tested samples.6-Gingerol presented a good linearity with peak area between the concentration of 0.024 8 and 0.124 mg/ml(r=0.996 6,n=5).The average recovery was 99.20% with the RSD of 1.26%.Conclusions] GC-MS was quick,simple,convenient and effective to analyze the composition of ethanol extracts.HPLC method was simple,reliable and reproducible to determine the content of 6-gingerol.