脱细胞血管基质的生物相容性研究

目的评估Triton X-100和胰蛋白酶方法制备的猪脱细胞血管基质的生物相容性。方法分别采用体外细胞毒性试验(直接接触法和MTT法)检测脱细胞血管基质的细胞毒性,采用皮下植入试验来评估经脱细胞血管基质的免疫排斥反应。结果L929细胞在预铺脱细胞血管基质的培养板内及不同浓度脱细胞基质浸提液内均生长良好,皮下植入试验显示2周后脱细胞血管基质的炎性反应明显减轻。结论经Triton X-100和胰蛋白酶方法制备的猪脱细胞血管基质具有良好的生物相容性。

Study for the Biocompatibility of Decellularized Vascular Scaffold

Objective To evaluate the biocompatibility of decellularized vascular scaffold prepared with Triton X-100 and trypsin. Methods In vitro and in vivo tests were performed to evaluate the biocompatibility of the decellularized vascular scaffold. The in vitro tests included direct contact method and MTT assay, and the in vivo test was subcutaneous implant test. Results L929 cells grew well on the glass slide covered with decellularized vascular scaffold and in the solution of decellularized vascular matrix. The patches implanted subcutaneously in SD mice only caused slight inflammatory reaction after 2 weeks. Conclusion Decellularized vascular matrices prepared with Triton X-100 and trypsin had a good biocompatibility.