| 白粉寄生孢ISSR-PCR体系的建立及遗传多样性的初步分析 |
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| 引用本文： | 赵云福,刘翠,梁晨,李宝笃. 白粉寄生孢ISSR-PCR体系的建立及遗传多样性的初步分析[J]. 菌物学报, 2010, 29(5): 653-664 |
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| 作者姓名： | 赵云福 刘翠 梁晨 李宝笃 |
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| 作者单位： | 1. 青岛农业大学农学与植物保护学院,青岛,266109;烟台市农业局植物保护站,烟台,262201
2. 青岛农业大学农学与植物保护学院,青岛,266109;山东省应用真菌重点实验室,青岛,266109 |
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| 基金项目： | 国家自然科学基金（No. 30870010）；山东省科技厅良种工程项目（No. 6208S5）；青岛农业大学校级项目（No. 630502） |
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| 摘 要： | 通过正交设计和单因素水平优化的方法对白粉寄生孢ISSR-PCR程序中的一些反应参数和ISSR引物进行优化和筛选,并利用建立的ISSR-PCR的反应体系来分析白粉寄生孢的遗传多样性。5个ISSR引物对73个菌株的扩增条带表明,ISSR标记在中国白粉寄生孢中存在较高的多态性,ISSR标记揭示白粉寄生孢的遗传多样性和寄主植物(真菌)多样性存在一定的相关性。
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| 关 键 词： | 重寄生菌 分子标记 引物 |
Establishment of ISSR-PCR system and preliminary analysis of genetic diversity for Ampelomyces quisqualis |
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| ZHAO Yun-Fu,LIU Cui,LIANG Chen and LI Bao-Du. Establishment of ISSR-PCR system and preliminary analysis of genetic diversity for Ampelomyces quisqualis[J]. Mycosystema, 2010, 29(5): 653-664 |
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| Authors: | ZHAO Yun-Fu LIU Cui LIANG Chen LI Bao-Du |
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| Affiliation: | Department of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, ChinaStation of Plant Protection, Yantai Municipal Agricultural Bureau, Yantai 262201, China;Department of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Key Laboratory of Applied Mycology, Qingdao 266109, China;Department of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Key Laboratory of Applied Mycology, Qingdao 266109, China;Department of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, ChinaShandong Key Laboratory of Applied Mycology, Qingdao 266109, China |
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| Abstract: | The reaction parameters and primers of ISSR-PCR for Ampelomyces quisqualis (Aq) were optimized by an orthogonal design and a single factor test. The genetic diversity of Aq was analyzed by the ISSR-PCR reaction system. Polymorphic bands of 73 isolates generated by five primers indicate that high diversity is conserved in Aq. The dendrogram based on ISSR results shows that the genetic diversity of Aq was relatively associated with the diversity of host fungi and host plants. |
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| Keywords: | hyperparasitic fungus molecular marker primer |
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