| 在唾液中检测幽门螺杆菌的意义 |
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| 引用本文： | 彭惠,潘国宗,曹世植.在唾液中检测幽门螺杆菌的意义[J].中华内科杂志,1999,0(3):171-173. |
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| 作者姓名： | 彭惠 潘国宗 曹世植 |
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| 作者单位： | 中国医学科学院 |
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| 摘 要： | 探讨一种有效的非侵入性幽门螺杆菌诊断方法。方法１３８／１５９例各类型胃、十二指肠疾病患者的唾液和胃黏膜，用空泡毒素基因为模板设计的引物行聚合酶链反应，其产物行单链构象多态性分析和Ｓｏｕｔｈｅｒｎ杂交。
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| 关 键 词： | 幽门螺杆菌 唾液 诊断 胃疾病 |
The significance of detection of Helicobacter pylori in saliva |
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| PENG Hui,PAN Guozong,CHAO Shizhi,et al..The significance of detection of Helicobacter pylori in saliva[J].Chinese Journal of Internal Medicine,1999,0(3):171-173. |
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| Authors: | PENG Hui PAN Guozong CHAO Shizhi |
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| Affiliation: | Department of Gastroenterology, Peking Union Medical College Hospital, Beijing 100730. |
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| Abstract: | Objective To explore noninvasive and effective method to detect Helicobacter pylori (Hp) in saliva. Methods DNAs of saliva and gastric mucosa from 138 and 159 patients of various gastro duodenal diseases were detected with PCR by use of primers amplifing vacA gene. The products were futher processed for SSCP and Southern blot hybridization. Results The rate of detection of Hp in saliva and gastric mucosa was 71.7% and 100% respectively. vacA 1 and vacA 2, being the two subtypes of vacA, were recognized in Hp . PCR/SSCP analysis showed a 98% identity of the strains of Hp in the saliva and gastric mucosa. Conclusion Hp could be colonized in saliva and probably transmited orally. The new method may provide noninvasive approach for detection of Hp and could be used in the investigation of family aggregation of Hp infection. |
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| Keywords: | Helicobacter pylori Saliva |
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