利用蛋白质组技术进行胃癌差异蛋白质研究

目的分离胃癌蛋白质组，找出胃癌组织的差异蛋白质点。方法利用二维聚丙烯酰胺凝胶电泳（2-DE）分离胃癌组织和正常对照组织的总蛋白质，并通过质谱分析技术对9个差异蛋白质点进行检测，以确定胃癌的差异蛋白质。结果在胃癌组织中点检测共有蛋白质点1147个，正常胃黏膜组织中点检测共有蛋白质点1079个。胃癌和正常胃黏膜组织两者共有164个差异蛋白质点，其中41个点仅在胃癌组织中表达，27个点仅在正常胃黏膜中表达；39个点在胃癌组织中高表达，57个点在胃癌组织中低表达。确定了7种在胃癌组织中明显高表达的蛋白质。结论利用蛋白质组技术获得了胃癌蛋白质组与正常对照组的差异蛋白质点，并获得了7种两者的差异蛋白质，为寻找胃癌的特异蛋白质奠定了基础。

Application of proteomics in the research of discrepancy proteins in gastric cancer

Objective To explore the discrepancy proteins in gastric cancer by proteome analysis. Methods Total proteins of gastric cancer tissues and matched normal gastric epithelial tissues were separated respectively by two-dimensional polyacrylamide gel electrophoresis (2-DE).Mass spectrometry was used to test the differentially expressed proteins.Results One thousand one hundred and forty-seven protein spots from gastric cancer tissue and 1079 spots from the normal tissue were gained.Out of 164 different protein spots,41 were only expressed in gastric cancer tissue,27 were unique in normal tissue,39 were up-regulated and 57 were down-regulated in gastric cancer.Seven proteins,which were highly expressed in gastric cancer tissue,were identified.Conclusion Different protein spots between gastric cancer tissues and normal gastric epithelial tissue were gained by proteomics.The 7 discrepancy proteins were further identified.It establishes the foundation of finding specific gastric cancer proteins,which act as biomarkers for the diagnosis and prognosis of gastric cancer.