多倍体萱草的离体快繁技术

以多倍体萱草的嫩叶、生长点、腋芽、花蕾、花瓣和花茎作外植体进行离体快繁技术研究。结果表明:以幼嫩的花蕾和花瓣为外植体最为适宜;以MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.7 mg/L为最适诱导培养基,诱导分化率可达74%以上;以MS+6-BA 2.0 mg/L+NAA 0.2 mg/L为最适继代增殖培养基,增殖系数可达5.8;以MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.02 mg/L+马铃薯20 g/L为壮苗培养基。培养1~2周后转入MS+NAA0.04 mg/L的生根培养基中,其平均根长、根长势等最为理想,生根率达100%,且移栽后成活率高,生长健壮。

Rapid in Vitro Propagation of Polyploid Hemerocallis fulva

The experiment was conducted to study the rapid in vitro propagation of polyploidy Hemerocallis fulva with tender leaf , growing point, axillary bud, bud, petal and scape as explants .The young bud and petal were the most suitable explants . MS+6-BA 0.5 mg/L+NAA 0.2 mg/L+2,4-D 0.7 mg/L was the most suitable inducing medium , and the inducing differen-tiation rate was 74%.MS+6-BA 2.0 mg/L+NAA 0.2 mg/L was the most suitable subculture medium , and proliferation co-efficient was 5.8.MS+6-BA 1.0 mg/L+NAA 0.2 mg/L+IBA 0.02 mg/L+Potato 20 g/L was the strong seedling medium . After 1-2 weeks, tissue culture seedling was transferred to the rooting medium of MS+NAA 0.04 mg/L.The average root length and root growth of tissue culture seedlings were the most ideal with the rooting rate of 100%, the survival rate was high, and the growth was strong .