视网膜光化学损伤大鼠视紫红质基因mRNA表达水平的原位杂交技术检测

目的：研究视网膜光化学损伤状况下视紫红质基因mRNA表达水平的变化规律及其与形态学改变的关系。 方法：应用原位杂交和电镜技术对光化学损伤大鼠视网膜视紫红质基因mRNA表达情况及视网膜形态学损伤性改变进行动态观察。 结果：视紫红质mRNA原位杂交信号主要分布于视网膜光感受细胞层,尤其是其内段和外段;随连续光照时间的延续,其表达迅速下降,且先于视网膜形态学的损伤性改变;同一视网膜中,上方及后极部杂交信号的减弱较下方和周边部更为明显。 结论：视紫红质基因mRNA表达水平降低可能为光化学性视网膜损伤的早期信号。 （中华眼底病杂志,1997,13：228-230）

THE CHANGES OF EXPRESSION LEVEL OF RHODOPSIN mRNA IN LIGHTDAMAGED RAT RETINAS THROUGH THE TECHNIQUE OF IN SITU HYBRIDIZATION

PURPOSE:The changes of expression level of rhodopsin mRNA and its relationship with the morphology in light damaged rat retinas were studied. METHODS:The changes of expresson level of rhodopsin mRNA in light damaged rat retinas and the changes on retinal morphology were observed through the technique of in situ hybridization and electron microscopy. RESULTS:The hybridization signals of rhodopsin mRNA mainly distributed in the photoreceptor layer of retina,relatively strong in the inner and outer segments. As the increase of light exposure time,the expression level of rhodopsin
mRNA in retinas greatly decreased before the changes on morphological injury of retina. For the same eye globe of the same rat at the same time,the hybridization signals at the upper and posterior region of the retina decreased more obviously than the lower and peripheral region of the retina. CONCLUSIONS:It was demonstrated for the first time that the expression of
rhodopsin mRNA was located at the photoreceptor layer of the retina. Continuous exposure to light could greatly decrease the expression of rhodopsin mRNA and the decreases differ regionally. It might be the early signals of retinal photic injury.It is a good method to study the expression level of retina mRNA through the in situ hybridization. （Chin J Ocul Fundus Dis,1997,13: 228-210）