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角膜缘干细胞培养体系的研究进展
引用本文:屈雷,杨学义,马勇江,华进联,窦忠英.角膜缘干细胞培养体系的研究进展[J].眼科新进展,2003,23(4):289-292.
作者姓名:屈雷  杨学义  马勇江  华进联  窦忠英
作者单位:712100,陕西杨凌,西北农林科技大学,陕西省干细胞工程技术研究中心
基金项目:国家自然科学基金(编号:39970363),国家重点基础研究发展规划基金(编号:G1999054300)~~
摘    要:正常角膜上皮形态稳定和功能的维持是依靠角膜缘干细胞的不断增殖和移行来实现。近年来,角膜缘干细胞的重要作用逐渐被人们所认识。本文从角膜缘干细胞的定位、体外取材、分离培养、培养条件的选择、常用体外培养的载体、鉴别方法等对角膜缘干细胞培养体系进行综述。

关 键 词:角膜缘  干细胞  培养体系
文章编号:1003-5141(2003)04-0289-04
修稿时间:2003年6月2日

Advances in the study of corneal limbal stem cells culture system
QU Lei,YANG Xue-Yi,MA Yong-Jiang HUA Jin-Lian,DOU Zhong-Ying From the Shaanxi Center of Stem Cell Engineering and Technology Northwest Sci-Tech University of Agriculture and Forestry,YangLing ,Shaanxi Province,China.Advances in the study of corneal limbal stem cells culture system[J].Recent Advances in Ophthalmology,2003,23(4):289-292.
Authors:QU Lei  YANG Xue-Yi  MA Yong-Jiang HUA Jin-Lian  DOU Zhong-Ying From the Shaanxi Center of Stem Cell Engineering and Technology Northwest Sci-Tech University of Agriculture and Forestry  YangLing  Shaanxi Province  China
Affiliation:QU Lei,YANG Xue-Yi,MA Yong-Jiang HUA Jin-Lian,DOU Zhong-Ying From the Shaanxi Center of Stem Cell Engineering and Technology Northwest Sci-Tech University of Agriculture and Forestry,YangLing 712100,Shaanxi Province,China
Abstract:Up to now, research workers have recognized that the normal corneal epithelial morphology and function are depended on continuously proliferation and migration of corneal limbal stem cell. There are some progressives results have been accumulated. This review focus on localization of limbal stem cells in vivo and how to get research materials to cultivate them, the choice of culture conditions, the carrier in use .At the same time, introduces the different methods of corneal limbal stem cell in vitro.
Keywords:corneal limbus  stem cell  culture system  
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