老年男性尿路感染病原菌生物膜检测及耐药性分析

目的探讨老年男性尿路感染的病原菌分布以及产生物膜阴性及阳性病原菌在耐药性上的差异,为临床使用抗菌药物提供依据。方法对358例老年男性尿路感染患者中段尿进行培养,对培养出的致病菌进行鉴定、药敏试验以及生物膜检测,并对产生物膜阴性及阳性病原菌的药敏结果进行对比统计。结果共分离出362株病原菌,以革兰阴性杆菌为主,占69.89%,革兰阳性球菌次之,占20.44%,真菌和其他病原菌占9.67%,革兰阳性球菌生物膜的检出率为40.54%,革兰阴性杆菌生物膜的检出率为41.11%,分离的主要病原菌为大肠埃希菌、凝固酶阴性葡萄球和变形菌属,分别占53.87%、15.75%、7.74%,其生物膜检出率分别为41.03%、45.61%、39.29%;生物膜检出阳性病原菌的耐药率高于生物膜检出阴性的病原菌,两者差异有统计学意义(P<0.01)。结论引起老年男性尿路感染的本研究以大肠埃希菌、凝固酶阴性葡萄球和变形菌属为主;耐药率较高的抗菌药物为氨苄西林、磺胺甲噁唑/甲氧苄啶、头孢呋辛以及青霉素;生物膜检出阳性病原菌的耐药率高于生物膜检出阴性的病原菌。

Biofilm detection of Pathogenic Bacteria in Old Male Urinary Infections and analysis of drug resistance

OBJECTIVE The study aims to discuss the distribution of pathogenic bacteria in old male urinary infections and the difference in the drug resistance of pathogenic bacteria with and without boifilm,and provide basis for clinical antibacterial drugs.METHODS Middle-segment urine were cultivated from 358 old male patients.The cultivated pathogenic bacteria were identified.Drug sensitivity test and biofilm detection were also carried out.The drug susceptibility results of pathogenic bacteria with and without biofilm were compared statistically.RESULTS Totally 362 pathogenic bacteria were isolated,among which the predominant pathogens were gram-negative bacteria,accounting for 69.89%,followed by gram-positive bacteria,accounting for 20.44%,fungi and other pathogens accounting for 9.67%.The biofilm detection rate of gram-positive bateria was 40.54%,and that of gram-negative bacteria was 41.11%.The isolated pathogens were mainly Escherichia coli,coagulase-negative Staphylococcus and Proteus,accounting for 53.87%,15.75% and 7.74%,respectively,and the biofilm detection rate were 41.03%,45.61%,39.29% respectively.The resistant rate of the pathogens with boifilm was higher than that without biofilm(P<0.01).CONCLUSION This study indicated that the old male urinary infection was mainly caused by E.coli,Coagulase-negative Staphylococcus and Proteus.The antibiotics with higher resistant rate are ampicillin,sulfamethoxazole/trimethoprim,cefuroxime axetil and penicillin.The resistant rate of pathogenic bacteria with biofilm is far higher than those without biofilm.