离子对反相高效液相色谱法测定人血浆中吉西他滨的药物浓度

 目的　建立使用离子对反相高效液相色谱测定吉西他滨血药浓度的方法。方法　血浆用三氯乙酸沉淀后取上清液直接进样。色谱柱为Symmetry C₁₈(4.6mm×250mm ,5μm) ,流动相为0.52%的磷酸二氢钠溶液(pH2.66)-乙腈(85:15,含0.202%庚烷磺酸钠)。柱温25℃,流速1.0mL·min^-1,检测波长273nm。结果　吉西他滨血药浓度在 0.1～100μg·mL^-1内的线性关系良好(r=0.9999),血浆中低、中、高3种浓度的方法回收率在97.39%～103.11%之间,日内RSD≤1.94%,日间RSD≤7.34%。结论　本法快速、灵敏、高效,适于吉西他滨的血药浓度监测。

Determination of gemcitabine in human plasma by ion-pair RP-HPLC

OBJECTIVE To establish a ion-pair RP-HPLC method for the determination of gemcitabine in human plasma. METHODS Samples were prepared by protein precipitation. The column was Waters Symmetry C₁₈(4.6mm×250mm,5μm) and the temperature was set at 25℃.The mobile phase was 0.52% phosphate buffer(pH2.66)-acetonitrile(85∶15) containing 0.202% sodium 1-heptanesulfonate and the flow rate was 1.0 mL·min^-1.The detection wavelength was at 273 nm.RESULTS The range of calibration curve was 0.1～100μg·mL^-1 (r=0.9999).The recovery of low, medium and high concentration were within 97.39%～103.11%.The RSD of intra-run and inter run did not exceed 1.94% and 7.34% respectively.CONCLUSION The method is rapid, sensitive and efficient.