| 灵发素对三七胚状体发生及其成苗的影响 |
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| 引用本文： | 许鸿源,蒙爱东,李春霞,邓锡青,周歧伟,杨美纯. 灵发素对三七胚状体发生及其成苗的影响[J]. 中药材, 2004, 27(10): 711-712 |
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| 作者姓名： | 许鸿源 蒙爱东 李春霞 邓锡青 周歧伟 杨美纯 |
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| 作者单位： | 1. 广西大学农学院,南宁,530005
2. 广西药用植物园,南宁,530001 |
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| 摘 要： | 以MS为基本培养基,加入适量的2,4-D和灵发素(LFS),于暗处培养,三七茎段愈伤组织可诱导出胚状体,2～3个月内发生机率可达90%左右.MS 2,4-D 1.5 mg/L LFS 2mg/L于光下培养,约有30%以上的胚状体能发育成健壮的全苗.
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| 关 键 词： | 灵发素 三七 胚状体 试管苗 |
| 修稿时间： | 2004-03-08 |
Effects of Lingfasu on Embryoid and Plantlet Formation of Panax notoginseng in Vitro |
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| Xu Hongyuan,Meng Aidong,Li Chunxia,Deng Xiqing,Zhou Qiwei,Yang Meichun. Effects of Lingfasu on Embryoid and Plantlet Formation of Panax notoginseng in Vitro[J]. Journal of Chinese medicinal materials, 2004, 27(10): 711-712 |
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| Authors: | Xu Hongyuan Meng Aidong Li Chunxia Deng Xiqing Zhou Qiwei Yang Meichun |
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| Affiliation: | Agricultural College, Guangxi University, Nanning 530005. |
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| Abstract: | On the medium MS added the right amount of 2,4-D and LFS (Angustmycin) and cultured under dark condition, the callus from stem segments of Panax notoginseng could induce a lot of embryoids. In 2-3 months, the ratio of embryoid formation reached about 90%. Then transplanted on MS + 2,4-D 1.5 mg/L + LFS 2 mg/L and cultured under light 20001x, near 30% embryoids could grow and develop as robust plantlets. |
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| Keywords: | Lingfasu (LFS Angustmycin) Panax notoginseng Embryoid Plantlet |
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