藏药湿生扁蕾对人结肠癌SW480细胞凋亡调控因子Bcl-2及Bax的mRNA表达的影响

目的：探讨藏药湿生扁蕾对SW480细胞凋亡调控因子Bcl-2mRNA及BaxmRNA表达的影响.方法：SW480细胞接种于6孔培养板中,24 h后分别以不同浓度（250、500、1000 μg/ml）的湿生扁蕾干预,继续培养24h后,提取每组总RNA,以GAPDH和β-actin基因为内参,采用RT-PCR法检测Bcl-2及Bax的mRNA表达水平.结果：Bcl-2 mRNA表达随湿生扁蕾浓度增加而减少,而Bax mRNA表达随湿生扁蕾浓度增加而增加,并呈现明显的剂量依赖关系.结论：藏药湿生扁蕾干预SW480细胞凋亡与其抑制Bcl-2的表达和促进Bax的表达有关.

The Effect of Tibetan Medicine Gentianopsis Paludosa About Colon Cancer SW480 Cell Apoptosis Regulatory Factors mRNA Expression of Bcl-2 and Bax

Objective： Discuss the effect of Tibetan medicine gentianopsis paludosa for SW480 cell apoptosis regulatory factors Bcl -2mRNA and BaxmRNA. Methods： Inoculate SW480 cell in 6holes culture plate. Intervene in gentianopsis paludosa using different concentration （250, 500, 1000 μg/ml） after 24h. Continue to develop 24h, and extract each group total RNA. Use GAPDH and β - actin gene as internal reference. Then adopt RT - PCR assay to detect mRNA expression level of Bcl - 2 and Bax. Results ： Bcl - 2mRNA expression are reducing with gentianopsis paludosa concentration increasing. While BaxmRNA expression is increasing with gentianopsis paludosa concentration increasing, and it presents the obvious dose dependency. Conclusion： Tibetan medicine gentianopsis paludosa control SW480 cells proliferation. It is connected with inhibition of Bcl -2 expression and promoting Bax expression.