| 环孢素A抑制正常人外周血记忆CD4~+T细胞产生IL-22的实验研究 |
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| 引用本文： | 沈二霞,杨宝欣,李旸,周茂华. 环孢素A抑制正常人外周血记忆CD4~+T细胞产生IL-22的实验研究[J]. 热带医学杂志, 2011, 11(8): 886-888,938 |
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| 作者姓名： | 沈二霞 杨宝欣 李旸 周茂华 |
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| 作者单位： | 1. 广州医学院病原生物学与免疫学教研室,广东广州,510182
2. 广东省人民医院检验科,广东省医学科学院,广东广州510080 |
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| 基金项目： | 广东高校优秀青年创新人才培育项目(LYM10111); 广州市属高校科研项目(10A159); 广东省卫生厅项目(A2009042) |
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| 摘 要： | 目的探讨环孢素A(CsA)是否能够抑制正常人CD4+T细胞产生白细胞介素22(IL-22)。方法使用密度梯度离心法制备正常人外周血单个核淋巴细胞(PBMCs),加入PMA+Inomycin刺激细胞后,做细胞内细胞因子染色,流式细胞仪检测正常人外周血PBMCs中产生IL-22的T细胞亚群,以及IL-17-IFN-γ-IL-22+CD4+T细胞亚群(Th22);PMA+Inomycin刺激正常人PBMCs,同时加或不加环孢素A(CsA)后,做细胞内细胞因子染色,流式细胞仪检测IL-22+CD4+T细胞和IFN-γ+CD4+T细胞的比例,以及IL-22+CD4+T细胞表达记忆表型分子CD45RO情况。结果正常人外周血中产生IL-22的T细胞以CD4+T细胞为主,且存在仅分泌IL-22,不分泌IL-17和IFN-γ的Th22亚群;CsA以剂量依赖方式抑制CD4+T细胞产生IL-22和IFN-γ;CsA抑制外周血PBMCs中记忆CD4+T细胞产生IL-22。结论 CsA能抑制正常人外周血中记忆CD4+T细胞产生IL-22。
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| 关 键 词： | 白细胞介素22 Th22 环孢素A |
CsA inhibits the production of IL-22 by memory CD4~+ T cells of PBMCs from healthy donors |
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| SHEN Er-xia,YANG Bao-xin,LI Yang,ZHOU Mao-hua. CsA inhibits the production of IL-22 by memory CD4~+ T cells of PBMCs from healthy donors[J]. Journal Of Tropical Medicine, 2011, 11(8): 886-888,938 |
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| Authors: | SHEN Er-xia YANG Bao-xin LI Yang ZHOU Mao-hua |
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| Affiliation: | SHEN Er-xia1,YANG Bao-xin1,LI Yang1,ZHOU Mao-hua2 (1.Department of Microbiology and Immunology,Guangzhou Medical College,Guangdong,Guangzhou 510182,2.Department of Laboratory Medicine,Guangdong General Hospital,Guangdong Academy of Medical Sciences,Guangzhou 510080,China) |
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| Abstract: | Objective To evaluate whether cyclosporine A(CsA) could inhibit the production of IL-22 by CD4+ T cells of PBMCs from healthy donors.Methods Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood of healthy donors using ficoll-hypaque density gradient centrifugation,and were stimulated with PMA+ionomycin.Intracellular staining and flow cytometric analysis was done to determine:IL-22 produced by CD4+ T,CD8+ T cells,IL-22+CD4+ T cells and Th22 cells of PBMCs.PBMCs were stimulated with... |
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| Keywords: | IL-22 Th22 cyclosporine A |
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