| 温敏性水凝胶对蛋白质和酶浓缩分离性能 |
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| 引用本文： | 王锦堂,仲慧,朱红军,张维光. 温敏性水凝胶对蛋白质和酶浓缩分离性能[J]. 陶瓷科学与艺术, 1998, 0(2) |
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| 作者姓名： | 王锦堂 仲慧 朱红军 张维光 |
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| 作者单位： | 南京化工大学应用化学系 |
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| 摘 要： | 报道了温敏性水凝胶———交联聚Ｎ异丙基丙烯酰胺（ＰＮＩＰＡＭ）的合成方法，研究了它对蛋白质和酶的浓缩分离性能。结果表明，ＰＮＩＰＡＭ凝胶对蛋白质和酶的分离效率在相转变温度附近发生突跃，显示出很好的浓缩分离能力。当ＰＮＩＰＡＭ凝胶中交联剂（Ｂｉｓ）质量百分数大于４％时，能实现高的分离效率。
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| 关 键 词： | 温敏性水凝胶 分离效率 蛋白质 酶 |
STUDY ON THE SEPARATION PROPERTY OF THE TEMPERATURE SENSITIVE GEL |
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| Wang Jintang Zhong hui Zhu Hongjun Zhang Weiguang. STUDY ON THE SEPARATION PROPERTY OF THE TEMPERATURE SENSITIVE GEL[J]. Ceramics Science & Art, 1998, 0(2) |
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| Authors: | Wang Jintang Zhong hui Zhu Hongjun Zhang Weiguang |
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| Affiliation: | Wang Jintang Zhong hui Zhu Hongjun Zhang Weiguang Department of Applied Chemistry,Nanjing University of Chemical Technology,Nanjing,210009,China |
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| Abstract: | The synthetic method of the temperature sensitive gel of cross linked poly N isopropylacrylamide (PNIPAM) and its separation property for biomacromolecules was studied.The experimental result showed that PNIPAM gel had excellent separation property for protein and enzyme. The separation efficiency happened abruptly around the phase transition temperature. The high separation efficiency can be accomplished when the concentration of cross linked reagent (Bis) >4% in PNIPAM gel. |
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| Keywords: | temperature sensitive gel separation efficiency protein enzyme |
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