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The use of monoclonal antibodies for detecting the influenza virus
Authors:AA Bakova  BD Brondz  OA Fedorkina  VA Gusev  AI Gordienko  IuS Krivoshein  IuL Krivorutchenko  TG Prokopenko  IA Popov
Abstract:The possibilities of using influenza A (Leningrad) 385/80 (H3N2) virus matrix protein-specific FITC-labeled D8 monoclonal antibodies in immunofluorescence assays were investigated. The virus antigen accumulation was detected in chorioallantoic cells of chick embryos. Exhibiting the type-specific properties, the fluorescent antibodies stain the perinuclear space, cytoplasmic membrane, and granular structures in the cytoplasm of infected cells. The haemagglutination test tires in the corresponding specimens were at least 1:16.
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