加味玉女煎对糖尿病胃肠功能紊乱大鼠血浆胃动素、血清胃泌素的实验研究

目的：探讨中药加味玉女煎对糖尿病胃肠功能紊乱大鼠血浆胃动素、血清胃泌素的影响,探讨其对改善作用的可能机制。方法：将实验大鼠采用链脲佐菌素和不规律喂养法复合诱导大鼠糖尿病胃肠功能紊乱模型后,用单日上午进食,双日下午进食,打乱大鼠进食规律,造成胃肠功能紊乱模型,随机分成中药组、西药组、生理盐水组3组,各10只。第4周起,开始治疗2周。第5周末麻醉处死,测血清胃泌素、血浆胃动素。结果：（1）模型组大鼠与空白组大鼠血清胃泌素存在显著性差异（P〈0.01）,加味玉女煎组大鼠血浆胃泌素含量明显低于模型组（P〈0.05）,与莫沙比利组比较无明显差异（P〉0.05）。（2）模型组大鼠血浆胃动素明显高于空白组（P〈0.01）,加味玉女煎组大鼠血浆胃动素含量明显低于模型组（P〈0.05）,与莫沙比利组比较无明显差异（P〉0.05）。结论：加味玉女煎可降低糖尿病胃肠功能紊乱大鼠血浆胃动素水平,血清胃泌素水平增加胃肠推进。

Experimental Study on the Effects of Modified Yunu Decoction on the Plasma Motilin and Serum Gastrin of Gastrointestinal Dysfunction Diabetic Rat

Objective：To discuss the therapeutic effect of Modified Yunu Decoction on pneumogastric neuropathy of the diabetes rats by observing its influence on motilin、gastrin and to disclose the mechanism of improving and treating diabetic gastrointestinal dysfunction.Methods：The diabetic rats were induced by IP of STZ and were fed irregularly.30 Wister diabetic rats were randomly divided into three groups：model group,Chinese medicine treatment group,Mosapride treatment group,10 rats in each group.The control group was fed regularly on standard rats feed.The other three groups were fed irregularly,eating in odd-number days and fasting in even-numbered days.Each group was perfused stomach with drugs during establishing the model,once per day,lasted 2 weeks.Fifth weekend anesthesia death was operated.We measured the levels of MOT and GAS in the blood with radioimmunoassay（RIA）.Result：（1）The influence on gastrin in blood serum：The model group and control group,there is a significant difference（P〈0.01）.The Modified Yunu Decoction group is obviously lower compared with model group（P〈0.05）;There is no remarkable difference between the Modified Yunu Decoction group and the Mosapride group（P〉0.05）.（2）The influence on the plasma motilin：MOT of model group is significantly higher than that of control group.Compared with model group,plasma motilin of the Modified Yunu Decoction group was significantly lower（P〈0.05）.There is no difference between the Modified Yunu Decoction group and the Mosapride group on the level of MOT in the blood（P〉0.05）.Conclusion：The findings suggested that Modified Yunu Decoction may have a value in preventing and treating of diabetic gastrointestinal dysfunction.