高原低氧环境对大鼠肺组织影响 |
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引用本文: | 李文华,袁东亚,孙芳云,李扬,张敏,巩江,宋土生,刘利英,程彦斌,黄辰.高原低氧环境对大鼠肺组织影响[J].中国公共卫生,2012,28(7):945-948. |
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作者姓名: | 李文华 袁东亚 孙芳云 李扬 张敏 巩江 宋土生 刘利英 程彦斌 黄辰 |
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作者单位: | 1. 西藏民族学院医学院高原环境与疾病相关基因研究重点实验室财政部与地方高校共建生命科学基础实验室,陕西咸阳,712082 2. 西安交通大学医学院环境与疾病相关基因教育部重点实验室 |
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基金项目: | 西藏自治区重点科研基金 |
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摘 要: | 目的观察高原低氧对大鼠肺组织超微结构影响及其低氧诱导因子1α(HIF-1α)表达变化。方法将SD大鼠分别进行高原低氧干预1、2、3和30 d,并设置对照组;4个高原低氧组由海拔5 m的西安地区途中耗时1d带到海拔2 700 m青海格尔木地区、途中耗时2 d带到海拔5 000 m唐古拉地区,途中耗时3d带到海拔4 500 m的西藏那曲地区,并饲养30 d。结果光镜及电镜观察显示,急性高原低氧2 d组肺组织出现明显高原肺水肿,高原低氧30 d组HIF-1αmRNA条带积分吸光度值(0.874±0.075)明显高于对照组(0.225±0.026)(P<0.01),高原肺水肿现象则明显减轻。结论低氧习服后肺组织低氧诱导因子1αmRNA表达升高,有利于减轻高原肺水肿。
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关 键 词: | 低氧诱导因子1α 高原习服 超微结构 |
Lung tissue ultrastructure and hypoxia-inducible factor 1 alpha expression of lung tissue in rats exposed to high altitude hypoxia |
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Affiliation: | LI Wen-hua*,YUAN Dong-ya,SUN Fang-yun,et al(*Key Laboratory of Genes Related to Plateau Enviroment and Disease,Medical School,Tibet Institute of Nationalities,Xianyang 712082,China) |
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Abstract: | Objective To examine the effects of high altitude hypoxia on ultrastructure and hypoxia-inducible factor 1α(HIF-1α) expression of lung tissue in the rats with high altitude hypoxia exposure.Methods Forty SD rats were exposed to high altitude hypoxia for 1,2,3,and 30 days,respectively,and a control group(10 rats) was set.The 4 altitude hypoxia groups were transported from the place of 5 m above sea level to the place of 2 700 m above sea level within one hour,to the place of 5 000 m above sea level within 2 hours,and to the place of 4 500 within 3 hours and raised at the place for 30 days.Results Light microscopy and electron microscopy showed that in 2 days acute high altitude hypoxia group there was apparent high altitude pulmonary edema.For the rats with high altitude hypoxia of 30 days,HIF-1α mRNA band integral absorbance values(0.874±0.075) was significantly higher compared with the control group(0.225±0.026,P<0.01) and high-altitude pulmonary edema was significantly reduced.Conclusion Hypoxic acclimatization could increase the expression of hypoxia-inducible factor 1α mRNA and reduce the high altitude pulmonary edema of lung tissue in rats. |
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Keywords: | hypoxia-inducible factor 1α plateau acclimatization ultrastructure |
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