速度赛马粪便微生物种群差异的研究

为筛选提高赛马竞技成绩的有益微生物,试验比较现役纯血速度赛马粪便微生物群落多样性及丰度差异.选择休赛一周的现役纯血马9匹,按比赛成绩分为A (16.8 m/s)、B(16.4 m/s)、C(16.3m/s)3组.收集新鲜粪便样本,提取粪便样本菌群DNA,对微生物16S rDNA的V3～V4区序列Illumina测序.结...

Study on the differences of microbial populations in the digestive tract of speed horses

In order to screen beneficial microorganisms that can improve the performance of horse racing,the experiment compared the diversity and abundance of the fecal microbial community of active purebred race horses.Choose nine purebred horses in active week of off-season,and divide them into three groups of A(16.8 m/s),B(16.4 m/s)and C(16.3 m/s)according to the competition results.Collect fresh fecal samples,extract fecal samples to extract bacterial group DNA,and sequence Illumina sequence of V3~V4 region of microorganism 16S rDNA.The experimental results show that there is a significant difference in the number of class and species classification microflora between group A and group C fecal microflora(P<0.05).The relative abundance distributions of the three groups of horse manure microbial marker species Bacteria,Firmicutes,Clostridia,Clostridiales,Veillonellaceae,and Anaerovibrio in group A from high to low are group A,group B,and group C.The LDA effect of Anaerovibrio in group A and Rhizobiales in group C was significant(P<0.05)According to the microbial abundance in horse dung,there are 4,3,5,9,and 14 groups of microbial differences in classes,orders,families,and genera,respectively.The conclusion of the study is that the digestive tract microbial preparations that improve horse racing performance can be screened according to the differences in microbial communities.