GC法测定芫荽茎叶中4种挥发性成分的含量

目的建立一种芫荽茎叶中芳樟醇、癸醛、正癸醇和十一醛的GC含量测定方法。方法以挥发油提取器蒸馏制备供试液;HP-5MSI毛细管色谱柱(30 m×0.25 mm,0.25μm),以氮气为载气,FID检测器,采用程序升温,内标法测定(柠檬烯为内标物)含量。结果芳樟醇、癸醛、正癸醇和十一醛进样质量浓度分别在31.5～315.0 mg.L-1(r=0.999 6)、269.0～2 690.0 mg.L-1(r=0.999 7)、95.3～953.0 mg.L-1(r=0.999 6)、58.1～581.0 mg.L-1(r=0.999 7)内呈良好线性关系,平均回收率分别为102.0(RSD=1.5%)、102.0(RSD=0.6%)、101.9(RSD=1.1%)、102.6(RSD=0.9%)。结论所建立的方法可用于芫荽茎叶中4种挥发性成分含量测定。

Determination of four volatile component in leaf of coriandrum sativum L. by GC

Objective To establish a method for determining the content of linalool, decanal, n-decanol, undecanal in coriander leaf. Methods The sample solution was distilled in vaporizing oil determination apparatus. With HP-5MSI capillary column(30 m×0.25 mm, 0.25 μm), nitrogen as the carrier gas, FID as the detector, the programmed temperature—GC and internal standard (Limonene was used for the internal standard) method were employed to determine the components. Results The results showed that the linear ranges of linalool, decanal, n-decanol and undecanal were 31.5-315.0 mg·L^-1 (r=0.999 6) , 269.0-2 690.0 mg·L^-1 (r=0.999 2), 95.3-953.0 mg·L^-1 (r=0.999 8), 58.1-581.0×10² mg·L^-1 (r=0.999 7).The average recovery of the method were 102.0% (RSD=1.5%), 102.0% (RSD=0.6%), 101.9% (RSD=1.1%), 102.6% (RSD=0.9%). The average recovery of the method were 102.0 (RSD=1.5%), 102.0 (RSD=0.6%), 101.9 (RSD=1.1%), 102.6 (RSD=0.9%). Conclusion The results show that this method is steady, accurate and practical which could be used for determining the linalool, decanal，n-decanol，undecanal in coriander leaf.