蕨麻提取物的体外抗HIV-1活性及毒性研究

目的研究蕨麻提取物的抗艾滋病病毒Ⅰ型(HIV-1)活性及毒性。方法用HIV-1实验室适应株SF33、临床分离株XJDC257和020100968、假病毒颗粒9-14,18-36,74-2和Z20-11分别感染TZM-bl、MT-4,利用基于TZM-bl细胞的荧光素酶检测体系和基于MT-4细胞的P24抗原检测方法,观察蕨麻提取物抑制HIV-1病毒复制的活性;用不同稀释度的蕨麻提取物与TZM-bl.MT-4.PBMCs共培养,使用Cell Counting Kit-8检测活细胞的数量,观察蕨麻提取物对相应细胞的毒性作用。结果利用MT-4细胞和TZM-bl细胞测得蕨麻提取物对SF33的半数抑制浓度(IC50)及选择性指数(SI)分别为6.2μg/mL,26.4和4.7μg/mL,73.5。蕨麻提取物对HIV-1临床分离株XJDC257和020100968的IC50和治疗指数(TI)分别为2.1μg/mL,70.6和1.9μg/mL,77.6;对HIV-1假病毒颗粒9-14、18-36、74-2和Z20-11的IC50分别为1.8μg/mL、1.0μg/mL、3.4μg/mL和3.5μg/mL,TI分别为81.9、147.5、43.4和42.1。结论蕨麻提取物在体外具有一定的抑制HIV-1复制活性。

Study on the anti-HIV-1 activity and toxicity of the potentilla anserine L. extract

Objective To study the anti-HIV-1 activity and toxicity of the potentilla anserine L.extract.Methods TZM-bl and MT-4 cell lines were infected with HIV-1 laboratory-adapted strain(SF33),two HIV-1 clinical isolates(XJDC257,020100968)and four HIV-1 pseudotype viruses(9-14,18-36,74-2 and Z20-11).The activity of inhibiting replication of HIV-1 was observed respectively by using Luciferase assay system based on TZM-bl cell line and P24 antigen expression detection based on MT-4 cell line..The TZM-bl,MT-4 and PBMCs were co-cultured with potentilla anserine L.extract of different concentrations,then CCK-8 kit was used to detect the number of the living cells for evaluating the cytotoxicity of the extract.Results The IC50 and SI of the potentill anserine L.extract against SF33 was 6.2 μg/mL,26.4 by using MT-4 cell line and 4.7 μg/mL,73.5 by using TZM-bl cell line.The IC50 and TI of the extract against HIV-1 clinical isolates XJDC257 and 020100968 was 2.1 μg/mL,70.6 and 1.9 μg/mL,77.6,respectively.The IC50 of the extract against the HIV-1 pseudotype viruses 9-14,18-36,74-2 and Z20-11 was 1.8 μg/mL,1.0 μg/mL,3.4 μg/mL and 3.5 μg/mL,respectively and therapeutic index(TI) was 81.9,147.5,43.4 and 42.1,respectively.Conclusion:The potentilla anserine L.extract could inhibit the replication of HIV-1 in vitro.