诱导型一氧化氮合酶和内皮型一氧化氮合酶在胃癌中的表达关系及临床意义

目的检测诱导型一氧化氮合酶(iNOS)、内皮型一氧化氮合酶(eNOS)在小鼠正常胃组织及原位移植人胃癌组织SGC-7901中的表达情况,探讨严重联合免疫缺陷(SCID)小鼠胃癌不同阶段时iNOS和eNOS含量变化的相关性研究及临床意义。方法采用苏木精-伊红染色法进行检测。结果可见eNOS和iNOS在10只正常胃组织的相关性比较弱,30只胃癌组织中,25只iNOS表达较正常胃组织明显升高差异有统计学意义(83%和38%,P<0.05),iNOS与胃癌的发生发展有关系。25只胃癌组织中有17只eNOS表达略高于正常胃癌组织(57%和20%,P<0.05)。iNOS与eNOS在胃癌组织中的表达有相关性(P<0.01)。结论eNOS和iNOS之间可能存在相互作用共同参与了胃癌的发展转移及浸润。

The expression relationship and clinical significance of induction nitric oxid esynthase and endothelial nitric oxide synthase in stomach cancer

Objective To detect the expression of inducible nitric oxidesynthase(iNOS),endothelial nitric oxide synthasee(eNOS),gastric cancer tissue in normal gastric tissue and in-place transplant patients,SGC-7901,and to investigate the correlation between changes in iNOS and eNOS content at different stages of stomach cancer in SCID mice,and clinical significance.Methods The detection was carried out by sumu-I-red dyeing method.Results It can be seen that the correlation between eNOS and iNOS was weak in 10 cases of normal stomach tissue,and in 30 cases of gastric cancer tissue,there was a significant difference between 25 cases of iNOS expression significantly higher than normal stomach tissue(83%vs 38%,P<0.05),and iNOS was associated with the development of stomach cancer.In 17 of the 30 gastric cancer tissues,eNOS expression was slightly higher than that of normal gastric cancer tissue(57%vs 20%,P<0.05).iNOS is associated with the expression of eNOS in gastric cancer tissue(P<0.01).Conclusion There may be interactions between eNOS and iNOS that are involved in metastasis and immersion for the development of stomach cancer.