维生素K2联合苯那普利对裸鼠胃癌的治疗作用及其机制的研究

 目的 通过建立胃癌裸鼠种植瘤模型观察维生素（Vit）K₂联合苯那普利对人胃癌SGC-7901裸鼠种植瘤的影响，以探讨两者对胃癌细胞的作用及机制。方法 通过人胃癌SGC-7901细胞接种建立胃癌裸鼠种植瘤模型后，随机分成对照组、Vit K₂组、苯那普利组和联合组，每组10只，灌胃2周后处死；TUNEL法检测各组胃癌细胞凋亡情况，免疫组化法检测血管内皮生长因子（VEGF）、caspase 3的蛋白表达情况。结果 与对照组相比，药物组能明显抑制瘤体生长，而联合组较单独药物组抑制作用更明显(P

Effect of Vitamin K2 and Benazepril Combination Therapy on Gastric Cancer in Nude Mice

OBJECTIVE To investigate the effect of vitamin K2 combined with benazepril on human gastric cancer in nude mice.METHODS SGC-7901 human gastric cancer cells were implanted into BALB/c nude mice to establish the cancer model.Fourteen days after the subcutaneous implantation of cancer cells,40 nude mice were randomly divided into 4 groups: control group,vitamin K2 therapy group,benazepril therapy group and combination therapy group(vitamin K2+ benazepril).After 14 d,the tumor growth was evaluated,apoptosis indices were examined by TUNEL assay,and VEGF and caspase 3 expression were assessed by immunohistochemical staining.RESULTS Compared to the control group,tumor weights were decreased and apoptosis indexes was significantly increased in the therapy groups(P<0.05),the protein expression of caspase 3 were up-regulated,and VEGF were decreased in the therapy group(P<0.05),and all the changes above were more significant in combination therapy group than vitamin K2 or benazepril group(P<0.05).CONCLUSION The combination of vitamin K2 and benazepril enhances anti-tumor efficacy possibly through angiogenesis suppression and apoptosis induction.