| 高效液相色谱法测定面刺霜中甲硝唑、氯霉素的含量 |
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| 引用本文： | 罗东,王章阳,代青,冉光炳,彭蓓.高效液相色谱法测定面刺霜中甲硝唑、氯霉素的含量[J].中国药房,2004,15(4):241-242. |
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| 作者姓名： | 罗东 王章阳 代青 冉光炳 彭蓓 |
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| 作者单位： | 1. 第三军医大学西南医院药学部,重庆市,400038
2. 重庆沙坪坝区人民医院药剂科,重庆市,400036 |
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| 摘 要： | 目的:建立同时测定面刺霜中甲硝唑和氯霉素含量的方法。方法:采用高效液相色谱法,色谱柱为Diamonsil C18(250mm ×4.6mm,5μm),流动相为甲醇(?)水(50:50),检测波长为280nm.结果:甲硝唑、氯霉素在10.0～250.0μg/ml浓度范围内均有良好的线性关系,相关系数均为0.9 998,平均回收率分别为100.54％、100.53％,相对标准差分别为0.79％,0.56％ 结论:本方法分离度好、快速、简便,可用于含甲硝唑、氯霉素霜剂的质量控制。
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| 关 键 词： | 高效液相色谱法 甲硝唑 氯霉素 含量测定 |
| 文章编号： | 1001-0408(2004)04-0241-02 |
| 修稿时间： | 2003年7月16日 |
Simultaneous Determination of Metronidazole and Chloramphenicol in Comedo Cream by HPLC |
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| LUO Dong,WANG Zhangyang,DAI Qing,RAN Guangbing.Simultaneous Determination of Metronidazole and Chloramphenicol in Comedo Cream by HPLC[J].China Pharmacy,2004,15(4):241-242. |
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| Authors: | LUO Dong WANG Zhangyang DAI Qing RAN Guangbing |
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| Abstract: | OBJECTIVE: To establish a HPLC method for simultaneous determination of metronidazole and chloram. phcnicol in comedo cream. METHODS: The method included a Diamonsil C18 column (250mm × 4.6mm, 5μm) and methanol water(50 : 50,v/v)as the mobile phase and an ultraviolet detecting wavelength of 280nm .RESULTS:The calibralion curve was linear in a concentration range of ( 10 - 250)μg/ml of both metronidazole and chloramphenicol (r = 0.9 998) .The average recoveries of metronidazole and chloramphenicol were 100.53% , 100.53% and RSD were 0.79% , 0.56% respectively .
CONCLUSION:The method for the determination of metronidazole and chloramphenicol was rapid and convenient. It can be used for the quality control of metronidazole and chloramphenicol in cream. |
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| Keywords: | HPLC metronidazole chloramphenicol content determination |
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