大豆黄素对5／6肾切除大鼠肾脏保护作用的研究

目的 探讨大豆黄素对5／6肾切除大鼠肾脏的保护作用及其机制。方法将26只雌性SD大鼠分为3组：假手术组（S组）8只，5／6肾切除模型组（M组）8只，大豆黄素治疗组（D组）10只。对M组和D组大鼠建立5／6肾切除模型，D组给予大豆黄素15mg·kg^-1·d^-1灌胃，M组给予等量生理盐水灌胃，共8周。检测各组大鼠治疗前、后24h尿蛋白定量、血肌酐（SCr）、尿素氮（BUN）、血清总蛋白（TP）和清蛋白（Alb），观察肾组织病理学表现，检测肾组织纤维连接蛋白（FN）、Ⅳ型胶原和转化生长因子β1（TGFβ1）表达。结果与M组相比，D组24h尿蛋白量、SCr、BUN降低（P〈0．05）；肾小球硬化指数降低（P〈0．05）；肾组织FN和Ⅳ型胶原蛋白表达降低（P〈0．01）；TGF-β1mRNA及其蛋白表达降低（P〈0．05）。结论大豆黄素对5／6肾切除大鼠肾脏有保护作用，可能与其抑制肾组织TGF-β1的表达有关。

The renal protective effects of daidzein on 5/6 nephrectomized rats

Objective To observe the renal protective effects of daidzein on 5/6 nephrectomized rats. Methods Twenty-six rats were randomly divided imo three groups： sham group（group S, n = 8）, 5/6 nephrectomized group（group M, n = 8） and daidzein treatment group（group D, n = 10）. 5/6 nephrectomized model was established in group M and group D. Biochemical indicators were measured before and 8 weeks after operation, pathological were observed, the renal expression of type Ⅳ collagen and fibronectin protein were observed. The renal expression of transforming growth factor beta1 （TGF-β1） protein and mRNA were also detected. Results Compared with group M, the urinary albumin excretion, serum creatinine and blood urea nitrogen in group D were decreased （P〈0. 05 ）, the glomerulosclerosis index was decreased （P% 0. 05 ）, the renal expression of type Ⅳ collagen and fibronectin protein were decreased（P〈0. 01 ）, the renal expression of TGF-β1 protein and mRNA were reduced were decreased（P〈0. 05）. Conclusion Daidzein have the renal protective effects on 5/6 nephrectomized rats.