GJB2通过AMPK/mTOR信号通路促进宫颈癌细胞恶性增殖

目的 筛选宫颈癌中具有临床意义的潜在药物靶点并研究此靶点蛋白在宫颈癌发生发展中的功能和潜在分子机制.方法 收集2018年7月至2019年7月济南市人民医院妇科行宫颈癌手术的30例宫颈癌患者肿瘤组织标本及癌旁组织标本,通过临床数据库分别找到在宫颈癌中高表达的基因(tumor/normal>2)以及在宫颈癌中具有显著临床预...

GJB2 promotes malignant proliferation of cervical cancer cells through the AMPK/mTOR signaling pathway

Objective To screen clinically significant potential drug targets in cervical cancer and study the function and potential molecular mechanisms of this target protein in the development of cervical cancer.Methods From July 2018 to July 2019,tumor tissue specimens and adjacent tissue specimens of 30 cases of cervical cancer patients undergoing cervical cancer surgery in the Gynecology Department of Jinan People’s Hospital were collected.According to the clinical database GEPIA,genes that are highly expressed in cervical cancer(tumor/normal>2)and genes that have significant clinical prognostic correlation in cervical cancer(pValue<0.05)were found.The overlapping analysis of these two genes was performed to find target genes with significant clinical significance,and the top 4 genes with the most significant difference in expression compared to normal tissues in cervical cancer were found through heat map analysis,and further screened by the principle of high expression and poor prognosis.Knocked down the expression of the target gene through siRNA,used cell proliferation experiments and cell migration experiments to verify the importance of the target gene in the development of cervical cancer.Finally,by analyzing the signal pathways involved in co-expressing genes with the target gene,the potential molecular mechanism of the target gene’s function in cervical cancer was explored,and further verified by Western blot experiments.Results A total of 78 genes were highly expressed in cervical cancer and have significant clinical prognosis;GJB2 was significantly highly expressed in cervical cancer,and patients with high expression had a poor prognosis;Knockdown of GJB2 inhibited the growth,proliferation and migration of cervical cancer cells;GJB2 may participate in the occurrence and development of cervical cancer through the AMPK/mTOR signaling pathway.Conclusion GJB2 overexpression inhibits AMPK phosphorylation and increases mTOR phosphorylation,thereby participating in the AMPK/mTOR signaling pathway to promote cervical cancer cell growth,proliferation and migration.