黄海北部真核微藻粒级结构及环境关联

采用高通量测序-分子鉴定分级技术于2019年对黄海北部海域真核微藻粒级结构进行了研究.结果发现，春季以中、小粒级为主，夏季以小、大粒级为主，秋季以大粒级为主，春、夏、秋季小、中、大粒级微藻比例为39：51：11、40：24：36、26：13：62.小粒级微藻优势种为细小微胞藻和金牛微球藻，中粒级微藻优势种为剧毒卡尔藻，大粒级微藻优势种为柔弱几内亚藻、平野亚历山大藻、多纹膝沟藻，综合整个真核微藻群落，春季由中粒径的剧毒卡尔藻占据优势（21.3%），夏季由大粒径的平野亚历山大藻占据优势（23.6%），秋季由大粒径的多纹膝沟藻占据优势（56.6%），有毒甲藻在该海域中占有绝对优势，贝毒累积风险较高，小粒径微藻中金牛微球藻和抑食金球藻曾在渤海引发褐潮，潜在威胁黄海北部贝类养殖业.

Characteristics of eukaryotic microalgae and their environmental correlation in the northern Yellow Sea

The size fractions of eukaryotic microalgae in northern Yellow Sea were studied by high-throughput sequencing and molecular identification classification technology in 2019. The results showed that the size fractions of eukaryotic microalgae were dominated by medium and small in spring, small and large in summer, and large in autumn. The proportions of small-sized, medium-sized and large-sized eukaryotic microalgae were about 39:51:11, 40:24:36, and 26:13:62 in spring, summer and autumn respectively. Micromonas pusilla and Ostreococcus tauri were the dominant species of small-sized eukaryotic microalgae, Karlodinium veneficum was dominant in medium-sized eukaryotic microalgae, and Guinardia delicatula, Alexandrium hiranoi, and Gonyaulax polygramma were dominant in large-sized of microalgae. In general, Karlodinium veneficum (medium-sized microalgae) were the dominant species in spring (21.3%), Alexandrium hiranoi (large-sized microalgae) in summer (23.6%) and Gonyaulax polygramma (large-sized microalgae) in autumn (56.6%). Toxic dinoflagellate was dominant in eukaryotic microalgae in northern Yellow Sea, which may cause high risk of shellfish poisoning toxins accumulation. Brown tide causative species Ostreococcus tauri and Aureococcus anophagefferens, small-sized microalgae, posed potential threat to shellfish aquaculture in northern Yellow Sea.