在海藻酸钠凝胶上诱导骨髓间充质干细胞分化为成骨细胞

通过在海藻酸钠凝胶上诱导bMSCs向成骨细胞分化，探讨其对骨髓间充质干细胞（bone mesenchymal stem cells, bMSCs）的生物学效应。采用MTT、甲苯胺蓝染色、von Kossa染色和RT-PCR分别检测细胞的增殖、生长形态、诱导后细胞的钙化结节和成骨相关基因的表达。实验组bMSCs生长状况良好、细胞增殖迅速，与对照组的增殖无差异；bMSCs成集落样生长明显，集落中央细胞重叠生长形成钙化结节；培养至12d，实验组和对照组的成骨相关基因，包括碱性磷酸酶、I型胶原和骨钙素，均为阳性表达，但实验组的表达量高于对照组。海藻酸钠凝胶能够促进bMSCs向成骨细胞的分化，是良好的骨组织工程支架材料。

Induction of Bone Mesenchymal Stem Cells to Form Osteoblast on Alginate Gels

Bio-material is a key factor in bone tissue engineering. The biological effects of a new bio-material, alginate gels, on bone mesenchymal stem cells (bMSCs) were investigated. bMSCs were induced to form osteoblasts on alginate gels. Cell growth, appearance and calcification were detected by MTT, toluidine blue stain and von Kossa stain, respectively. Osteoblast related genes were examined by RT-PCR. It is found that bMSCs on the alginate gels (experimental group) grew well and formed obvious colony. The genes related with osteoblast (alkaline phosphatase, collagen I and osteocalcin) were positive both in the experimental group and the control group (bMSCs on tissue culture plastic), moreover, the expression levels in the experimental group were higher than those in the control group. The results show that alginate gels can promote bMSCs to differentiate into osteoblasts. This suggests that alginate gels may act as a favourable scaffold in bone tissue engineering.