| 反相高效液相色谱-增敏化学发光同时测定VB1和VC |
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| 引用本文： | 王莉,周光明,邓传跃,黄成,游水英,沈祥,高智席.反相高效液相色谱-增敏化学发光同时测定VB1和VC[J].分析科学学报,2006,22(5):533-536. |
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| 作者姓名： | 王莉 周光明 邓传跃 黄成 游水英 沈祥 高智席 |
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| 作者单位： | 西南大学化学化工学院,重庆,400715 |
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| 摘 要： | 本文基于维生素B1(VB1)、维生素C(VC)对鲁米诺(Luminol)和铁氰化钾(K3Fe(CN)6])化学发光反应的增敏作用原理,建立了反相高效液相色谱(RP-HPLC)分离、柱后化学发光检测VB1与VC的新方法。当在鲁米诺中加入KBr时可以大大增敏该反应。本法已成功运用于蜂蜜和复合维生素片中VB1与VC的测定。
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| 关 键 词： | 高效液相色谱 化学发光 维生素B1 维生素C |
| 文章编号： | 1006-6144(2006)05-0533-04 |
| 收稿时间： | 2005-05-25 |
| 修稿时间： | 2005-06-21 |
Determination of Thiamin and Ascobic Acid by Reversed-Phase High Performance Liquid Chromatography with Chemiluminescence Detection |
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| WANG Li,ZHOU Guang-ming,DENG Chuan-yue,HUANG Cheng,You Shui-ying,SHEN Xiang,GAO Zhi-xi.Determination of Thiamin and Ascobic Acid by Reversed-Phase High Performance Liquid Chromatography with Chemiluminescence Detection[J].Journal of Analytical Science,2006,22(5):533-536. |
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| Authors: | WANG Li ZHOU Guang-ming DENG Chuan-yue HUANG Cheng You Shui-ying SHEN Xiang GAO Zhi-xi |
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| Affiliation: | School of Chemistry and Chemical Engineering, Southwest China Normal University,Chongqing 400715 |
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| Abstract: | A novel high performance liquid chromatograph chromatography(HPLC)-Chemiluminescence(CL) detection method for simultaneous determination of VB_1 and VC based on the chemiluminescence reaction of thiamin(VB_1) and ascorbic acid(VC) with Luminol-FeK_3(CN)_6-KBr system was developed.The proposed method has been applied to the determination of VB_1 and VC in a pharmaceutical and honey preparation. |
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| Keywords: | High performance liquid chromatography Chemiluminescence Thiamin Ascorbic acid |
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