小碎斑鱼蛉幼虫肠道细菌分离及鉴定研究

目的从微生态学角度研究小碎斑鱼蛉幼虫的营养生理活动。方法从小碎斑鱼蛉幼虫肠道环境中进行分离、纯化和培养,获得3个细菌菌株,对其菌体形态、染色反应、培养性状和生理生化反应进行系统研究。结果上述3个菌株均属于芽胞杆菌属(Bacillus),1号菌株为巨大芽胞杆菌(Bacillus megaterium),2号菌株为枯草芽胞杆菌(Bacillus subtilis),3号菌株为地衣芽胞杆菌(Bacillus licheniformis)。结论小碎斑鱼蛉幼虫肠道环境中的不同细菌,其数量存在明显差异。

Isolation and identification of intestinal bacteria in Neochauliodes sparsus larvae

Objective The study the nutritional physiological activities of Neochauliodes sparsus larvae from a microecological perspective.Method Three bacteria strains were isolated from the intestines of the larvaes.The bacterial morphology,staining reaction,cultural characters,physiological and biochemical reactions were studied systematically.Result The three strains were identified as belonging to Bacillus,of which the No 1 strain was Bacillus megaterium,the No 2 was Bacillus subtilis,and the No 3 was Bacillus licheniformis.Conclusion The quantities of different bacteria vary significantly in intestinal environment of the larvae of Neochauliodes sparsus.