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镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析
引用本文:邓志瑞,罗琳. 镉离子胁迫下4种辣椒超氧化物歧化酶-同工酶酶谱及酶活性分析[J]. 上海大学学报(自然科学版), 2009, 15(2): 216-220
作者姓名:邓志瑞  罗琳
作者单位:(上海大学 生命科学学院,上海 200444)
基金项目:上海市高等学校科学技术发展基金 
摘    要:以4种辣椒为材料,分析在不同胁迫浓度和胁迫时间镉离子胁迫下,4种辣椒超氧化物歧化酶(SOD)活性及同工酶酶谱的变化.结果表明,镉离子能显著影响酶活性的变化,不同镉离子浓度胁迫下不同材料酶活性变化不同, SOD酶活性最高为对照组的1.4倍(1号品种,100 mg/L胁迫3 d),酶活性最低的为对照组的60%(2号品种,150 mg/L胁迫6 d);镉离子还能影响同工酶酶谱,不同胁迫时间和浓度对同工酶的影响不同,对1号材料的影响最为显著,能诱导出2条SOD同工酶新谱带.

关 键 词:辣椒;镉;超氧化物歧化酶(SOD);同工酶;酶谱;酶活性  
收稿时间:2007-11-06

Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd2+ Stress
DENG Zhi-rui,LUO Lin. Catalytic Activity and Isozyme Patterns of Superoxide Dismutase in Four Species of Capsicums under Cd2+ Stress[J]. Journal of Shanghai University(Natural Science), 2009, 15(2): 216-220
Authors:DENG Zhi-rui  LUO Lin
Affiliation:(School of Life Sciences, Shanghai University, Shanghai 200444, China)
Abstract:Catalytic activity and isozyme patterns of superoxide dismutase (SOD) were studied in four species of capsicums at different times under series concentration of cadmium (Cd). The results show that Cd2+ can affect catalytic activity of SOD remarkably and dissimilarly according to its concentration. The highest catalytic activity is 1.4 times higher compared to the control (No.1 species, 100 mg/L for 3 d) while the lowest is only 60% of that of control
(No.2 species, 150 mg/L for 6 d). Besides, Cd2+ can also affect isozyme patterns of SOD, especially in No.1 species. Two new isozyme bands are induced.
Keywords:capsicums   cadmium   superoxide dismutase(SOD)   isoenzyme   isozyme patterns   catalytic activity,
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