KCO1 is a component of the slow-vacuolar (SV) ion channel |
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Authors: | Schönknecht Gerald Spoormaker Petra Steinmeyer Ralf Brüggeman Lioubov Ache Peter Dutta Rajiv Reintanz Birgit Godde Matthias Hedrich Rainer Palme Klaus |
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Affiliation: | Julius-von-Sachs-Institut für Biowissenschaften, Lehrstuhl für Molekulare Pfanzenphysiologie und Biophysik, Universit?t Würzburg, 97082 Würzburg, Germany. |
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Abstract: | The Arabidopsis double pore K+ channel KCO1 was fused to green fluorescent protein and expressed in tobacco protoplasts. Microscopic analysis revealed a bright green fluorescence at the vacuolar membrane. RT-PCR experiments showed that KCO1 is expressed in the mesophyll. Vacuoles from Arabidopsis wild-type and kco1 knockout plants were isolated for patch-clamp analyses. Currents mediated by slow-activating vacuolar (SV) channels of mesophyll cell vacuoles were significantly smaller in kco1 plants compared to the wild-type. This shows that KCO1 is involved in the formation of SV channels. |
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