高速逆流色谱法从多花蒿中分离制备阿格拉宾

目的 采用高速逆流色谱法分离制备多花蒿中阿格拉宾.方法 多花蒿乙醇提取物经硅胶柱色谱分离,所得组份Fr.1用高速逆流色谱进一步分离,采用正己烷-醋酸乙酯-甲醇-水(1.2∶0.8∶1.5∶0.5)为两相溶剂系统,上相为固定相,下相为流动相,主机转速850 r/min,体积流量2.0 mL/min,检测波长为254 nm.采用波谱法对所得化合物进行结构鉴定,HPLC法测定产品的纯度.结果 从500 mg组分Fr.1中得到260 mg质量分数为99.5％的阿格拉宾.结论 该方法操作简单,可用于阿格拉宾化学对照品的分离制备.

Separation and preparation of arglabin from Artemisia myriantha by high speed counter-current chromatography

Objective To establish a method for the isolation and preparation of arglabin from Artemisia myriantha by high speed counter-current chromatography(HSCCC).Methods The ethanol extract of A.myriantha was separated with silica gel column chromatography,and the obtained Fr.1 was further separated by HSCCC with a two-phase solvent system composed of n-hexane-ethylacetate-methanol-water(1.2:0.8:1.5:0.5).The lower phase was used as mobile phase,and the upper phase was used as the stationary phase.Main engine speed was 850 r/min,and the detection wavelength was 254 nm at a flow rate of 2.0 mL/min.The obtained fraction was identified by spectral analysis and analyzed by HPLC.Results A total of 260 mg arglabin with the purity of 99.5% was successfully obtained from 500 mg Fr.1.Conclusion The results indicate that HSCCC is a simple and rapid method for the separation and preparation of arglabin reference substance.