Effect of Different Factors on in Vitro Maturation of Porcine Oocytes

Effects of several factors such as exogenous hormones and ITS,culture methods,paraffin oil on in vitro maturation (IVM) for porcine oocytes were studied.The results indicated that,the group supplemented with PMSG+HCG+FSH was significantly higher than FSH+LH and HMG in mature media with regard to the mature rates of porcine oocytes (80.1% vs 68.3% vs 53.3%,P<0.05);the mature rate of porcine oocytes cultured in mature media supplemented with 1% ITS was not improved,while the cleavage rate and blastocyst rate of parthenogenesis embryos were significantly increased (63.3% vs 55.1%,18.7% vs 12.1%,P<0.05);the groove dish group was significantly higher than four-hole plate and 30 mm plastic dish group in terms of the mature rates of porcine oocytes (73.3% vs 68.0% vs 68.3%,P<0.05);mature media supplemented with paraffin oil was significantly increased the cumulus expansion and maturation rates for porcine oocytes (84.8% vs 69.9%,P<0.05). The results showed that the mature rates of porcine oocytes cultured in groove dish,supplemented with paraffin oil,PMSG+HCG+FSH and ITS were significantly improved.