| 叶下珠复方Ⅱ号对HSC—T6增殖及TIMP—lmRNA表达的影响 |
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| 引用本文： | 盛扬,李常青,刘妮,赵妨.叶下珠复方Ⅱ号对HSC—T6增殖及TIMP—lmRNA表达的影响[J].中国热带医学,2008,8(2):204-205. |
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| 作者姓名： | 盛扬 李常青 刘妮 赵妨 |
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| 作者单位： | 广州中医药大学热带医学研究所,广东广州,510405 |
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| 基金项目： | 广州中医药大学校科研和教改项目 |
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| 摘 要： | 目的观察叶下珠复方Ⅱ号对肝星状细胞（HSC～T6）增殖及基质金属蛋白酶抑制因子mRNA（TIMP—lmRNA）表达的影响，探讨叶下珠复方Ⅱ号抗肝纤维化效果和作用机理。方法体外培养HSC—T6，随机分为对照组和叶下珠复方Ⅱ号5．00、2．50和1．25mg／ml组。加药后用MTT法检测各组HSC—T6增殖变化，且采用RT—PCR方法测定各组HSC—T6的TIMP—lmRNA表达。结果叶下珠复方Ⅱ号各剂量组药物对HSC—T6的增殖有明显的抑制作用，且TIMP—lmRNA表达都低于正常对照组。结论叶下珠复方Ⅱ号能押制HSC—T6增殖和TIMP—lmRNA表达，从而减弱TIMP—l对基质金属蛋白酶的抑制作用，促进ECM的降解，这可能是叶下珠复方Ⅱ号抗肝纤维化的作用机制之一。
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| 关 键 词： | 叶下珠复方Ⅱ号 肝星状细胞 基质金属蛋白酶抑制因子-1 细胞外基质 增殖 |
| 文章编号： | 1009-9727(2008)2-204-02 |
| 修稿时间： | 2007年12月10 |
Impact of compound phyllanthus urinaria (CPU Ⅱ )on proliferation of HSC-T6 and expression of TIMP-1mRNA |
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| Affiliation: | SHENG Yang, LI Chang-qing, LIU Ni, et al. (Institute of Tropical Medicine ,Guangzhou University of Traditional Chinese Medicine ,Guangzhou 510405, Guangdong, P. R. China) |
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| Abstract: | Objective To observe the impact of compound phyllanthus urinaria （CPUII） on the proliferation and expression of TIMP - 1 mRNA of cultured hepatic stellate cells （ HSC - T6）, and explore the antJfibrotic mechanisms of CPU Ⅱ Methods HSC - T6 were cultured in vitro and divided into 4 groups randomly, including control group,CPU Ⅱ 5mg/ml group, CPU Ⅱ 2.5mg/ml group, CPU Ⅱ 1.25mg/ml group. After incubation with CPU Ⅱ , MTT colormetric assay was used to evaluate the inhibitive effect of CPUII on HSC - T6,and the expression of the TIMP- 1 mRNA were determined by using semi - quanititative RT- PCR method. Results The levels of proliferation of HSC - T6 and expression of T/MP- 1 mRNA in every treatment groups were significantly lower than that of control group. Condus/on CPUII is capable of inhibiting HSC - T6 proliferation and down - regulating the expression of TIMP - 1, thus the antifibrotic mechanism of CPU Ⅱ might be partly due to its downregulation of the expression of TIMP - 1 mRNA and acceleration of the degration of ECM in HSC - T6 cells, |
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| Keywords: | Compound Phyllanthus Urinaria Ⅱ （ CPU Ⅱ ） HSC - T6 tissue inhibitor of metalloproteinase 1 ECM Proliferation |
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