| 3H-TdR与125I-UdR掺入淋巴细胞增殖的比较 |
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| 引用本文： | 万建美,张友九,范我,朱然,宁萍,崔凤梅.3H-TdR与125I-UdR掺入淋巴细胞增殖的比较[J].中华放射医学与防护杂志,2010,30(6):658-660. |
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| 作者姓名： | 万建美 张友九 范我 朱然 宁萍 崔凤梅 |
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| 作者单位： | 苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室;苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室;苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室;苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室;苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室;苏州大学放射医学与公共卫生学院 江苏省放射医学与防护重点实验室 |
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| 基金项目： | 江苏省卫生厅医学科技发展基金(H200341);苏州市社会发展科技计划资助(SS0416) |
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| 摘 要： | 目的 比较 3H-TdR与 125I-UdR掺入淋巴细胞的增殖效应。方法 用 3H-TdR与 125I-UdR掺入法测定淋巴细胞和Daudi淋巴瘤细胞的增殖效应。结果 3H-TdR和 125I-UdR在正常淋巴细胞中的掺入率分别为20.95%±1.06%和1.00%±0.04%,在Daudi淋巴瘤细胞中的掺入率分别为29.94%±4.10%和6.02%±0.73%。 3H-TdR在细胞中的掺入率明显高于 125I-UdR;且 3H-TdR和 125I-UdR在淋巴瘤细胞中的掺入率高于正常淋巴细胞。结论 就淋巴细胞而言,作为示踪剂 125I-UdR不能替代 3H-TdR;但对于淋巴瘤细胞,能否代替 3H-TdR有待于进一步研究。
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| 关 键 词： | 淋巴细胞 淋巴瘤细胞 3H-TdR 125I-UdR 掺入率 |
| 收稿时间： | 2010/7/13 0:00:00 |
Comparison of 3H-TdR and 125I-UdR incorporation on the proliferation effect of lymphocytes |
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| WAN Jian-mei,ZHANG You-jiu,FAN Wo,ZHU Ran,NING Ping and CUI Feng-mei.Comparison of 3H-TdR and 125I-UdR incorporation on the proliferation effect of lymphocytes[J].Chinese Journal of Radiological Medicine and Protection,2010,30(6):658-660. |
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| Authors: | WAN Jian-mei ZHANG You-jiu FAN Wo ZHU Ran NING Ping and CUI Feng-mei |
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| Affiliation: | School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China;School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China;School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China;School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China;School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China;School of Radiation Medicine and Public Health, Soochow University, Suzhou 215123, China |
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| Abstract: | Objective To compare the incorporation method of 3H-TdR and 125I-UdR on determining the proliferation effect of lymphocytes.Methods The proliferation effects of lymphocyte and Daudi lymphoma cells were estimated by 3H-TdR and 125I-UdR incorporation. Results The incorporating fraction of 3H-TdR and125I-UdR into lymphocyte was 20.95%±1.06% and 1.00%±0.04%, respectively, and the incorporating fraction for the lymphoma cells was 29.94%±4.10% and 6.02%±0.73%,respectively. The incorporation fractions of 3H-TdR into lymphocyte and lymphoma cells were much higher than those of 125I-UdR, but the incorporating fractions of 3H-TdR or 125I-UdR into the lymphoma cells were much higher than those of lymphocytes. Conclusions For lymphocytes, 125I-UdR cannot substitute 3H-TdR as a tracer agent. But for lymphoma cells, whether 125I-UdR could be replace 3H-TdR or not needs further research. |
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| Keywords: | Lymphocytes Lymphoma cells 3H-TdR 125I-UdR Incorporating fraction |
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