| RP-HPLC法测定板蓝根、大青叶中靛蓝、靛玉红的含量 |
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| 引用本文： | 孙立新,唐虹,尹萍,宁黎丽,毕开顺.RP-HPLC法测定板蓝根、大青叶中靛蓝、靛玉红的含量[J].沈阳药科大学学报,2000,17(3):191-193. |
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| 作者姓名： | 孙立新 唐虹 尹萍 宁黎丽 毕开顺 |
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| 作者单位： | 1. 沈阳药科大学药学系,沈阳,110015
2. 辽宁卫生职工医学院,沈阳,110015 |
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| 摘 要： | 用RP HPLC以安宫黄体酮为内标物同时测定板蓝根、大青叶中靛蓝、靛玉红的含量 .色谱条件 :色谱柱为SpherisorbC18柱 ;流动相为甲醇 水 (6 2∶38,V∶V) ;检测波长 2 80nm ;靛蓝和靛玉红的平均回收率分别为 (99 0± 1 1) %和 (10 4 2± 3 5 ) % .本法操作简便 ,结果准确
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| 关 键 词： | 板蓝根 大青叶 靛蓝 靛玉红 高效液相色谱法 |
| 修稿时间： | :1999-10-0 |
Determination of the Content of Indigo and Indirubin in the Indigowoad Root and Leaves by RP-HPLC |
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| Sun Lixin,Tang Hong,Yin Ping,Ning Lili,Bi Kaishun.Determination of the Content of Indigo and Indirubin in the Indigowoad Root and Leaves by RP-HPLC[J].Journal of Shenyang Pharmaceutical University,2000,17(3):191-193. |
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| Authors: | Sun Lixin Tang Hong Yin Ping Ning Lili Bi Kaishun |
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| Abstract: | An analytical method for determination of indigo and indirubin in the indigowoad root and leaves by RP HPLC was developed.The HPLC conditions were as follows:spherisorb C 18 column(4 6 mm×250 mm);the mobile phase,methanol H 2O(62∶38, V∶V) ;detection wavelength,280 nm;internal standard,medroxyprogesterone acetate.The mean recoveries for the indigo and indirubin were(99 0±1 1)% and(104 2±3 5)%,respectively.The method was simple and accurate. |
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| Keywords: | indigowoad root indigowoad leaves indigo indirubin HPLC |
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