| TNT荧光偏振免疫检测方法研究 |
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| 引用本文： | 陆文总,倪原,赵晨,张亮,任郁苗.TNT荧光偏振免疫检测方法研究[J].西安工业大学学报,2013(1):19-23. |
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| 作者姓名： | 陆文总 倪原 赵晨 张亮 任郁苗 |
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| 作者单位： | 西安工业大学电子信息工程学院 |
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| 基金项目： | 西安工业大学校长基金(XAGDXJJ1014) |
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| 摘 要： | 研究旨在利用新制备的多克隆抗体首次建立2，4，6-三硝基甲苯（TNT）的荧光偏振免疫分析方法．通过设计合成新的TNT半抗原TNT—BSA，偶联载体蛋白后免疫新西兰大白兔，制备亲和力高、特异性强的多克隆抗体．结合新设计合成的荧光示踪物TNT—PDAM建立了TNT的荧光偏振免疫分析方法．试验结果表明，TNT荧光偏振免疫分析方法检测限为7．5μg／L，检测范围为13．8~129．7μg／L,IC50为39．3μg／L．
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| 关 键 词： | TNT 荧光偏振 抗体 示踪物 |
Determination of TNT Using Fluorescence Polarization Immunoassay |
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| Authors: | LU Wen-zong NI Yuan ZHAO Chen ZHANG Liang REN Yu-miao |
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| Affiliation: | (School of Electronic and Information Engineering,Xi’an Technological University,Xi’an 710021,China) |
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| Abstract: | One fluorescence polarization immunoassay （FPIA） was developed for detecting 2, 4, 6- Trinitrotoluene （TINT） based on its polyclonal antibody. Animals were immunized by the synthesized artificial antigen and TNT specific antibody can be obtained. The fluorescence- labeled TNT was synthesized with 1-PyrenyldiazoMethane （PDAM） and purified by solid phase extraction （SPE）. The TNT FPIA had a dynamic range from 13. 8 to 129.7μg/L with IC50 value of 39.3 μg/L and the detection limit was 7. 5 μg/L. The FPIA method is of higher sensiticity, simplicity and stability,so it is a potential way to determine TNT. |
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| Keywords: | TNT tluorescence polarization immunoassay antibody tracer |
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