| 独角莲中酪氨酸酶活性抑制成分的提取工艺研究 |
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| 引用本文： | 宋艳玲,杜新春,庞颖,殷连保,张毅,戴宏斌.独角莲中酪氨酸酶活性抑制成分的提取工艺研究[J].沈阳化工学院学报,2014,28(3):231-233. |
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| 作者姓名： | 宋艳玲 杜新春 庞颖 殷连保 张毅 戴宏斌 |
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| 作者单位： | 沈阳化工大学制药与生物工程学院,辽宁沈阳,110142 |
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| 基金项目： | 辽宁省大学生科技创新项目，辽宁省教育厅项目 |
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| 摘 要： | 利用独角莲水提物对酪氨酸酶抑制活性的测试,研究独角莲水提物的提取工艺.通过L9(34)正交试验法研究了料液质量比、提取时间、提取温度和提取次数对独角莲水提物抑制酪氨酸酶活性的影响.结果表明:独角莲水提物的最佳提取工艺为料液质量比1∶15,提取时间3 h,提取温度80℃,提取次数1次,在此条件下测定独角莲水提物对酪氨酸酶的抑制率,最高为46.93%.
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| 关 键 词： | 独角莲 水提物 酪氨酸酶 |
Extraction Technology and Effect on Tyrosinase Activity of Water Extracts from Typhonium Giganteum Engl. |
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| SONG Yan-ling,DU Xin-chun,PANG Ying,YIN Lian-bao,ZHANG Yi,DAI Hong-bin.Extraction Technology and Effect on Tyrosinase Activity of Water Extracts from Typhonium Giganteum Engl.[J].Journal of Shenyang Institute of Chemical Technolgy,2014,28(3):231-233. |
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| Authors: | SONG Yan-ling DU Xin-chun PANG Ying YIN Lian-bao ZHANG Yi DAI Hong-bin |
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| Affiliation: | (Shenyang University of Chemical Technology, Shenyang 110042, China) |
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| Abstract: | The extraction technology of water extracts from Typhonium giganteum Engl. was studied and the effect on tyrosinase activity was investigated. The influence of material-liquid ratio, extraction time, extraction temperature and extraction times on inhibitory activity against tyrosinase was studied through L9 （ 34 ） orthogonal experiments. The effects on tyrosinase activity was dertermined by Folin-reagent method. The optimum extraction condition was material-liquid ratio 1：15 （weight ratio）, extraction time 3 h, extraction tempreture 80 ℃, and extraction for 1 time. At this condition, water extracts from Typhonium giganteum Engl. had the highest inhibitoty effects on tyrosinase, which was 46. 93 %. |
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| Keywords: | Typhonium giganteum Engl water extracts tyrosinase |
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