| 慢性乙型肝炎肝内HBV DNA和HBsAg的双标记定位研究 |
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| 引用本文： | 张晓东,王文亮,刘彦仿,王伯沄,郭冰芳.慢性乙型肝炎肝内HBV DNA和HBsAg的双标记定位研究[J].临床与实验病理学杂志,1993(Z1). |
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| 作者姓名： | 张晓东 王文亮 刘彦仿 王伯沄 郭冰芳 |
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| 作者单位： | 第四军医大学病理学教研室，第四军医大学病理学教研室，第四军医大学病理学教研室，第四军医大学病理学教研室，第四军医大学病理学教研室 西安710032 天津武警医专 300162，西安710032，西安710032，西安710032，西安710032 天津武警医专 300162 |
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| 摘 要： | 应用原位杂交和免疫组化PAP法双标记技术,结合病人乙型肝炎(乙肝)病毒血清学标志物检测结果,研究了31例慢性乙肝病人肝穿刺组织中乙型肝炎病毒DNA(HBV DNA)和HBsAg的分布及意义。结果显示,肝组织内检出HBV DNA 23例,HBsAg 26例,二者同时检出者21例。从同组病人肝组织内HBV DNA和HBsAg双标检测结果与其乙肝病毒血清学标志物检测结果的比较来看,肝组织内HBV DNA和HBsAg同时阳性很可能表明HBV正处于复制及表达的活动状态。
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| 关 键 词： | 慢性乙型肝炎 乙型肝炎病毒DNA 乙型肝炎表面抗原 杂交 免疫组织化学 |
Localization of HBV DNA and HBsAg in Liver with Chronic Hepatitis B by Double Labelled Method |
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| Zhang Xiaodong et al Dept of Pathol,The Fourth Military Medical University,Xian.Localization of HBV DNA and HBsAg in Liver with Chronic Hepatitis B by Double Labelled Method[J].Chinese Journal of Clinical and Experimental Pathology,1993(Z1). |
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| Authors: | Zhang Xiaodong Dept of Pathol The Fourth Military Medical University Xian |
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| Abstract: | The distribution and the signifi-cance of hepatitis B virus DNA(HBV DNA)and HBsAg in 31 cases of liver puncture tissueswith chronic hepatitis B were studied by doublelabelled technique of in situ hybridiazation andimmunohistochemical PAP method.The resultsof the study were analysed connecting with theserologic markers.The results showed that 23cases were positive for HBV DNA and 26 posi-tive for HBsAg and 21 pasitive for both HBVDNA and HBsAg.According to the results ofcomparing HBV DNA and HBsAg in the livertissues with the serologic markers of the samegroup patients,the positiveness of both HBVDNA and HBsAg in the liver tissues might likelyindicate that HBV were in a state of the replica-tion and the expression. |
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| Keywords: | chronic hepatitis B hepatitis B virus DNA HBsAg hybridzation immunohistochemistry |
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