基于微卫星标记的中国东北地区灰飞虱遗传变异及种群遗传结构分析

【目的】本研究旨在明确我国东北地区灰飞虱Laodelphax striatellus种群遗传变异及种群遗传结构,阐明种群间遗传分化与基因流。【方法】利用9对微卫星引物对采自我国东北地区15个地理种群的375头灰飞虱样品进行测序与分析;利用GeneAlex6.51, GENEPOP4.0.9和STRUCTURE 2.3.4等软件分析灰飞虱地理种群间的遗传多样性、遗传分化、基因流及种群遗传结构。【结果】在所分析的375头灰飞虱个体中,各位点平均有效等位基因数Na=6.898;总体上,灰飞虱不同地理种群遗传多样性较高(平均观测杂合度Ho=0.548;平均期望杂合度He=0.582),各种群间基因流较低(Nm=0.660)。UPGMA聚类树、PCoA及STRCTURE分析结果表明,东北地区灰飞虱种群分为两分支:吉林(JL)和沈阳(SY2012,SY2013和SY2014)种群聚为一支;其余种群聚为一支。AMOVA分析结果表明,灰飞虱遗传变异主要来自种群内(87%),种群间变异水平较低(13%)。【结论】中国东北地区灰飞虱遗传多样性较高,不同地理种群存在一定程度的遗传分化,且基因交流较低,存在一...

Genetic variation and population genetic structure of the small brown planthopper,Laodelphax striatellus(Hemiptera: Delphacidae), in Northeast China based on microsatellitemarkers

【Aim】 The purpose of this study is to clarify the population genetic variation,population genetic structure, population genetic differentiation and gene flow of the smallbrown planthopper, Laodelphax striatellus, in Northeast China. 【Methods】 Nine pairs ofmicrosatellite primers were used to sequence and analyze 375 samples of L. striatelluscollected from 15 geographical populations in Northeast China, and the genetic diversity,genetic differentiation, gene flow and population genetic structure of these geographicalpopulations were analyzed using GeneAlex 6.51, GENEPOP 4.0.9 and STRUCTURE 2.3.4 based onthe nine microsatellite (SSR) molecular markers. 【Results】 The average number ofeffective alleles per locus was 6.898 among the 375 individuals of L. striatellus assayed.In general, the genetic diversity of different geographical populations of L. striatelluswas high (mean observed heterozygosity Ho=0.548; mean expected heterozygosity He=0.582),and the gene flow between different populations was low (Nm=0.660). The results of UPGMAclustering tree, PCoA and STRCTURE analysis showed that L. striatellus populations inNortheast China were divided into two groups: populations from Jilin (JL) and Shenyang(SY2012, SY2013 and SY2014) were clustered into one group, and the remaining populationsinto another group. The AMOVA analysis showed that the genetic variation of L. striatelluswas mainly from within the population (87%), and the inter-population variation level waslow (13%). 【Conclusion】 The genetic diversity of L. striatellus in Northeast China isrelatively high, different geographical populations have a certain degree of geneticdifferentiation, the gene exchange is low and a certain population genetic structureexists.