海藻酸-磷脂微囊复合水凝胶的制备、表征及毒理学分析

采用薄膜分散法合成磷脂微囊,根据胶粒的双电层理论,通过在微囊中加入氯化锰、氯化钙和氯化镁电解质溶液,使微囊处于相对稳定的状态.研究发现加入氯化锰和氯化钙溶液,微囊胶体的粒径没有明显的变化,但加入一定浓度氯化镁溶液,其粒径明显变大.为了进一步增加磷脂微囊稳定性,将氯化锰、氯化钙、氯化镁磷脂微囊胶体分别与海藻酸钠(SA)溶液混合.结果表明,氯化镁与SA几乎不能形成水凝胶,氯化钙与SA形成水凝胶能力强于氯化锰.微囊胶体溶液中的磷脂酰丝氨酸(PS)可以与Ca~(2+)和Mg~(2+)键合形成PS-Ca~(2+)和PS-Mg~(2+),但不能与Mn~(2+)键合形成PS-Mn~(2+).对氯化钙磷脂微囊与海藻酸钠合成的复合水凝胶的形貌、溶胀率及细胞毒性进行了表征,结果表明,氯化钙与SA形成的水凝胶可以捕获胶体中磷脂微囊,且形貌规整,结构稳定,无细胞毒性.

Preparation,Characterization and Toxicological Analysis of Alginate-phospholipid Vesicle Composite Hydrogels

Phospholipid vesicles were synthesized by thin-film dispersion.According to the electric double layer theory of micelles,the vesicles were stabilized by electrolyte solutions,including manganese chloride,calcium chloride and magnesium chloride.There are no significant changes in the particle sizes of the vesicles colloid by the additions of manganese chloride and calcium chloride solutions,respectively.However,the particle sizes of the vesicles were significantly bigger by adding a certain amount of magnesium chloride.To further increase the stability of the phospholipid vesicles,alginate-phospholipid vesical composite hydrogels were prepared by dropping sodium alginate(SA) solution into phospholipid vesicle colloids stabilized by manganese chloride,calcium chloride and magnesium chloride,respectively.The results show that magnesium chloride and SA hardly formed hydrogel,and calcium chloride and SA formed hydrogel much easier than manganese chloride did.The phosphatidylserine(PS) in the phospholipid vesicles bound to the Ca2+ and Mg2+ through forming PS-Ca2+,PS-Mg2+,respectively,however PS cannot interact with Mn2+,forming PS-Mn2+.The morphology,swelling ratio and cytotoxicity of the composite hydrogels made of alginate-phospholipid vesicles were characterized.The results show that a novel composite hydrogel could be prepared by calcium-crosslinking SA and the PS in the phospholipid vesicles colloid,forming stable and non-cytotoxic composite hydrogel.