四氧化三铁纳米粒子表面接枝聚L-谷氨酸苄酯多孔微载体的构建和性能

在氨基化改性的四氧化三铁(Fe₃O₄)纳米粒子表面引发N-羧酸酐L-谷氨酸苄酯(BLG-NCA)开环聚合, 制得四氧化三铁/聚L-谷氨酸苄酯(PBLG)复合材料(Fe₃O₄-g-PBLG). 改变单体和引发剂比例可以调控Fe₃O₄-g-PBLG的接枝率, 采用热失重分析(TGA)测得实际接枝率分别为66.36%, 79.66%和89.52%. 通过双乳液挥发法制备磁性Fe₃O₄-g-PBLG多孔微载体, 其中接枝率为89.52%的微载体密度为1.034 g/mL, 孔隙率为92.57%, 粒径为200~300 μm, 孔径为40~50 μm, 保水率为370%~400%. 同时Fe₃O₄-g-PBLG微载体具有超顺磁性, 在外加磁场作用下可排布成任意形状, 对治疗复杂结构的骨缺损具有显著优势. 因此Fe₃O₄-g-PBLG多孔微载体在骨组织工程领域具有潜在应用价值.

Fabrication and Performance for Fe3O4 Nanoparticles Surface Grafted Poly(γ-benzyl-L-glutamate) Porous Microcarriers†

Ferroferric oxide/poly(γ-benzyl-L-glutamate)(PBLG) composite materials(Fe3O4-g-PBLG) was synthesized by initiating γ-benzyl-L-glutamate-N-carboxyanhydride(BLG-NCA) polymerization on the surface of amino-functionalized ferroferric oxide(Fe3O4) nanoparticles.Fe3O4-g-PBLG composites was confirmed by Fourier transform infrared and X-ray diffraction.Thermogravimetric(TGA) study showed that the grafting ratios of Fe3O4-g-PBLG were 66.36%,79.66%,and 89.52%,and grafting ratio of Fe3O4-g-PBLG composite could be controlled.The Fe3O4-g-PBLG with grafting ratio of 89.52% was used to fabricate magnetic Fe3O4-g-PBLG porous microcarriers.The porous microcarriers possessed suitable density(1.034 g/mL),porosity(92.57%),particle size(200-300 μm),pore size(40-50 μm) and water retention(370%-400%).Meanwhile,microcarriers exhibited superparamagnetism.Under the action of magnetic fields,the microcarriers can be arranged into any shape,showing significant advantages for the treatment of complex structure of the bone defects.These results showed that Fe3O4-g-PBLG porous microcarriers might be applied in bone tissue engineering.