汉滩型与希望山型汉坦病毒的体外重组

目的：探明汉坦病毒汉滩型与希望山型之间能否发生巧克力理排，以及发生重排的频率和特点，方法：用汉坦病毒汉滩型76－118株与希望山型PHV株混合感染VeroE6细胞，空斑形成实验挑取子代病毒克隆株，传代培养后用RTPCR方法鉴定子代病毒基因型。结果：子代病毒14／36来源于PHV株；12／36来源于76－118株；5／36汉滩型与希望山型分型引物扩增均为阳性；1株子代病毒L，S片断均来源于76－118株，而M片段两种引物扩增均为阳怀；2株子代病毒L片断来源于76－118株，S片断来源于PHV株，M片段两种引物扩增均为阳性。1株子代病毒L，S片断均来源于76－118株，而M片段来源于PHV株，结论：汉坦病毒汉滩型与希望山型之间可以发生基因片段的重排。

Genetic reassortment between Hantaan virus strains and prospect hill virus strains

AIM To determine the frequency and characteristics of gene segments reassortment between Hantaan virus and Prospect Hill virus. METHODS Mixed infections were initiated in tissue culture by using Hantaan virus strains (76 118) and Prospect Hill Virus Strains (PHV). Potential reassortant virus plaques were screened by multiplex RT PCR, using primers specific for individual genome segments of each strain. RESULTS Most of the progeny virus plaques (28 of 36) had the parental genotype of 76 118 strain or PHV strain. 5 of 36 plaques had mixed genotypes that yielded RT PCR bands for the same segment of both parental strains. Reassortant viruses were detected in 4 of 36 progeny plaques tested. In addition, approximately 8.33% of the progeny virus plaques appeared to contain M segments originating from both parental virus strains, i.e., they were diploid. CONCLUSION Genetic reassortment can occur between Hantaan virus strains and Prospect Hill virus strains.