| 响应面分析法优化超声辅助提取红肉苹果花青苷工艺 |
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| 引用本文： | 窦玉慧,孙晓红,祝 军 等. 响应面分析法优化超声辅助提取红肉苹果花青苷工艺[J]. 中国酿造, 2014, 0(4): 65-70 |
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| 作者姓名： | 窦玉慧 孙晓红 祝 军 等 |
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| 作者单位： | [1]青岛农业大学园艺学院,山东青岛266109 [2]青岛农业大学生命科学学院,山东青岛266109 |
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| 基金项目： | 国家现代苹果产业技术体系项目（CARS-28-01-07） |
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| 摘 要： | 采用响应面分析法优化超声波辅助提取红肉苹果花青苷的提取工艺。以花青苷的含量作为响应值,在单因素试验基础上,根据Box-Behnken中心组合试验设计原理采用4因素3水平的响应面分析法对各因素进行分析,结果表明,红肉苹果花青苷的最佳提取条件为丙酮体积分数80%,料液比1∶14(g∶mL),超声时间32min,盐酸含量1.2%,在此条件下提取的花青苷的质量浓度为7.01mg/100mL,与响应面预测理论值(7.09mg/100mL)相当。
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| 关 键 词： | 红肉苹果 花青苷 工艺优化 响应面分析 |
Optimization of ultrasonic-assisted solvent extraction of anthocyanin from red-flesh apple by response surface methodology |
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| DOU Yuhui,SUN Xiaohong,ZHU Jun,ZHANG Yugang,DAI Hongyi. Optimization of ultrasonic-assisted solvent extraction of anthocyanin from red-flesh apple by response surface methodology[J]. China Brewing, 2014, 0(4): 65-70 |
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| Authors: | DOU Yuhui SUN Xiaohong ZHU Jun ZHANG Yugang DAI Hongyi |
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| Affiliation: | 1. College of Horticulture, Qingdao Agricultural University, Qingdao 266109, China; 2.College of Life Science, Qingdao Agricultural University, Qingdao 266109, China) |
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| Abstract: | Response surface methodology was used to optimize the process for the solvent extraction of anthocyanin from red-flesh apple with ultra- sonic assistance. Taking cyanine glucoside content as response value, on the basis of single factor experiment, Box-Behnken central composite design and four factors three levels response surface analysis theory to optimize the anthocyanin extraction process. Result showed that the optimum condi- tions for anthocyanin extraction were determined as follows： acetone concentration 80%, solid-liquid ratio 1：14 （g/ml）, extraction time 32 min, and acid concentration 1.2%. Under such condition, the concentration of anthocyanin was 7.01 mg/100 ml, close to the predicted value 7.09 mg/100 ml. |
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| Keywords: | red-flesh apple anthocyanin process optimization response surface methodology |
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