咖啡酸对辐射损伤小鼠造血恢复作用的初步研究

目的 观察咖啡酸对辐射损伤小鼠造血恢复的影响。方法 建立接受4.5 Gy X射线照射的辐射损伤小鼠模型,BALB/c小鼠随机分为空白对照组,单纯照射组,CFA组,观察小鼠30天存活率、体重、外周血白细胞(WBC)、血红蛋白(HGB)、血小板(PLT)、骨髓有核细胞(BMNC)、粒细胞巨噬细胞集落形成单位(CFU-GM)、巨核细胞形成单位(CFU-MK)及脾集落形成单位(CFU-S)和脾脏指数(SI)等指标。结果 CFA组小鼠的30天存活率、体重、WBC、BMNC、CFU-GM、CFU-MK、CFU-S和SI数均明显高于单纯照射组,差异有统计学意义(P < 0.05或P < 0.01)。结论 CFA能促进辐射损伤小鼠的造血恢复。

Preliminary Study on Hematopoietic Reconstruction Effects of Caffeic Acid in Lethally Irradiated Mice

Objective To investigate the hematopoietic reconstruction effects of caffeic acid(CFA) in lethally irradiated mice. Methods 42 BALB/c mice were randomly divided into 3 groups, 14 rats in each group:normal control group (no radiation, no filling medicine),irradiation group (radiation, no filling medicine), CFA group (administered CFA after irradiation). The mice in all groups except normal control group were irradiated with 4.5 Gy X-ray. The mice in CFA group were poured through gastic rote with CFA at the dose of 100mg/kg,once perday for 28 days. The survival rate of 30 days, body weight, the number of white blood cells(WBC),hemoglobin (HGB), platelet (PLT),bone marrow nuclear cells (BMNC), granulocyte-macrophage colony-forming unit (CFU-GM),colony-forming unit of megakaryocyte (CFU-MK),colony-forming unit-spleen (CFU-S),spleen index(SI) were observed. Results Survival rate,body weight, WBC, HGB, PLT, BMNC, CFU-GM, CFU-MK, CFU-S, SI counts were significantly higher in CFA group than those of the irradiation group(P < 0.01 or P < 0.05). Conclusion Caffeic acid could effectively reconstruct hematopoiesis in lethally irradiated mice.