| 高效液相色谱法检测乳制品中硫氰酸根含量 |
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| 引用本文： | 赵贞,吕海燕,刘丽君,万鹏,李翠枝,邵建波. 高效液相色谱法检测乳制品中硫氰酸根含量[J]. 乳业科学与技术, 2015, 38(6): 19-21. DOI: 10.15922/j.cnki.jdst.2015.06.005 |
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| 作者姓名： | 赵贞 吕海燕 刘丽君 万鹏 李翠枝 邵建波 |
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| 作者单位： | 内蒙古伊利实业集团股份有限公司,内蒙古呼和浩特,010010 |
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| 摘 要： | 建立乳制品中硫氰酸根的高效液相色谱分析方法.样品经乙腈沉淀蛋白,以磷酸二氢钠磷酸缓冲液-乙腈溶液为流动相,强阴离子交换(SAX)色谱柱分离,紫外检测器218 nm检测.结果表明:在硫氰酸根0.2～100 mg/kg范围内呈良好的线性关系,回收率为98.5％～113.0％.本方法用于乳制品中硫氰酸根的测定,操作简单、定量准确、稳定性好.
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| 关 键 词： | 硫氰酸根；液相色谱法；乳制品 |
Detection of Thiocyanatein in Dairy Products by High-Performance Liquid Chromatography |
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| ZHAO Zhen,L(U) Haiyan,LIU Lijun,WAN Peng,LI Cuizhi,SHAO Jianbo. Detection of Thiocyanatein in Dairy Products by High-Performance Liquid Chromatography[J]. JOURNAL OF DAIRY SCIENCE AND TECHNOLOGY, 2015, 38(6): 19-21. DOI: 10.15922/j.cnki.jdst.2015.06.005 |
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| Authors: | ZHAO Zhen L(U) Haiyan LIU Lijun WAN Peng LI Cuizhi SHAO Jianbo |
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| Affiliation: | (Inner Mongolia Yili Industrial Group Co. Ltd., Hohhot 010010, China)Abstract: A method for detecting thiocyanate in dairy products by high-performance liquid chromatography |
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| Abstract: | A method for detecting thiocyanate in dairy products by high-performance liquid chromatography (HPLC) isdescribed. The proteins in the samples were precipitated by the addition of acetonitrile, then the filtrate was separated on astrong anion exchange (SAX) column using a mobile phase made up of a mixture of sodium dihydrogen phosphate solutionand methanol. The target compound was detected with a UV detector at 218 nm. The results showed that the calibrationcurve displayed a good linear relationship in the range of 0.2–100 mg/kg. The recovery rate of thiocyanate was 98.5%–113.0%.The method is simple with good accuracy and stability for detecting thiocyanate in dairy products. |
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| Keywords: | thiocyanate high-performance liquid chromatography (HPLC) dairy products, |
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